SYSTEMIC LUPUS-ERYTHEMATOSUS PATIENTS WITH CENTRAL-NERVOUS-SYSTEM INVOLVEMENT SHOW AUTOANTIBODIES TO A 50-KD NEURONAL MEMBRANE-PROTEIN
SYSTEMIC LUPUS-ERYTHEMATOSUS PATIENTS WITH CENTRAL-NERVOUS-SYSTEM INVOLVEMENT SHOW AUTOANTIBODIES TO A 50-KD NEURONAL MEMBRANE-PROTEIN
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DOI：
10.1084/jem.176.2.565
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发表时间：
1992-08-01
影响因子：
15.3
通讯作者：
PUSZKIN, S
中科院分区：
文献类型：
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作者：
HANSON, VG;HOROWITZ, M;PUSZKIN, S
在系统性红斑狼疮（SLE）和中枢神经系统（CNS）受累患者血清中检测到一种抗体，该抗体与脑突触末梢质膜中的50-kD抗原反应。用Triton X-100从富含突触质膜的制剂中溶解50-kD抗原，并通过分子筛过滤柱色谱法部分纯化。20例CNS-SLE患者中有19例的血清与50-kD蛋白呈强至中度免疫反应性。免疫反应性与50-kD蛋白也检测到在脑脊液中的中枢神经系统系统性红斑狼疮患者。来自健康个体的对照血清不与50-kD蛋白反应。在一组无CNS表现的SLE患者中，35%的患者检测到低背景反应性，在3%的显示其他结缔组织疾病的患者中检测到低背景反应性。在这项研究中，共有100人接受了测试。从CNS-SLE患者纯化的50 kD蛋白的自身抗体用于神经母细胞瘤细胞的免疫荧光标记。免疫荧光染色显示细胞膜表面有明显的斑点状分布。两者合计，数据表明，50 kD蛋白可能是一个重要的目标，自身抗体，主要发现在CNS-SLE患者，抗原可能发挥作用的发病机制中的一些神经系统表现在SLE。
An antibody was detected in the sera from patients with systemic lupus erythematosus (SLE) and central nervous system (CNS) involvement that reacted with a 50-kD antigen in the plasma membrane of brain synaptic terminals. The 50-kD antigen was solubilized with Triton X-100 from preparations enriched with synaptic plasma membranes, and was partially purified by molecular sieve filtration column chromatography. The sera of 19 of 20 CNS-SLE patients showed strong to moderate immunoreactivity with the 50-kD protein in Western blots. Immunoreactivity with the 50-kD protein was also detected in the cerebrospinal fluid of CNS-SLE patients. Control sera from healthy individuals did not react with the 50-kD protein. Low to background reactivity was detected in 35% of a group of SLE patients without CNS manifestations, and in 3% of patients displaying other connective tissue diseases. A total of 100 individuals were tested in this study. Purified autoantibodies to the 50-kD protein from CNS-SLE patients were used for immunofluorescent labeling of neuroblastoma cells. The immunofluorescent staining revealed a distinct macular distribution pattern on the surface of the cell membrane. Taken together, the data suggest that the 50-kD protein may be an important target for autoantibodies, preponderantly found in CNS-SLE patients, and that the antigen may play a role in the pathogenesis of some neurological manifestations in SLE.