METALLOPROTEINASES FROM RABBIT BONE CULTURE-MEDIUM DEGRADE TYPE-IV AND TYPE-V COLLAGENS, LAMININ AND FIBRONECTIN
METALLOPROTEINASES FROM RABBIT BONE CULTURE-MEDIUM DEGRADE TYPE-IV AND TYPE-V COLLAGENS, LAMININ AND FIBRONECTIN
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DOI：
10.1042/bj1990807
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发表时间：
1981-01-01
影响因子：
4.1
通讯作者：
BURGESON, RE
中科院分区：
文献类型：
--
作者：
MURPHY, G;CAWSTON, TE;BURGESON, RE
从兔骨组织培养液中分离出3种具有抗胶原、抗蛋白多糖和抗明胶活性的金属蛋白酶。降解蛋白聚糖的级分还降解层粘连蛋白、纤连蛋白和胃蛋白酶溶解的IV型胶原的聚合产物，并且还可以溶解不溶性IV型胶原。降解明胶的级分能够降解溶解的V型和1 α型，2 α，3.alpha.（软骨）胶原蛋白，以及胃蛋白酶溶解的IV型胶原蛋白的低分子量产物。所有的活性都能被1，10-菲咯啉抑制，并以部分或全部潜伏的形式出现，可被4-氨基苯汞乙酸盐激活。
Gel-filtration chromatography of culture medium from rabbit bone explants separates 3 latent metalloproteinases with activities against collagen, proteoglycan and gelatin, respectively. The fractions degrading proteoglycan also degrade laminin, fibronectin and the polymeric products of pepsin-solubilized type IV collagen and can also solubilize insoluble type IV collagen. The fractions degrading gelatin are capable of degrading solubilized type V and 1.alpha.,2.alpha.,3.alpha. (cartilage) collagens, as well as the lower-MW products of pepsin-solubilized type IV collagen. All activities can be inhibited by 1,10-phenanthroline and occur in either partially or totally latent forms that can be activated by 4-aminophenylmercuric acetate.