Simultaneous enhancement of separation selectivity and solvent strength in reversed-phase liquid chromatography using micelles in hydro-organic solvents.
Simultaneous enhancement of separation selectivity and solvent strength in reversed-phase liquid chromatography using micelles in hydro-organic solvents.
复制标题
使用水有机溶剂中的胶束同时增强反相液相色谱中的分离选择性和溶剂强度。
DOI：
10.1021/ac00201a009
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发表时间：
1990
影响因子：
7.4
通讯作者：
Breyer,ED
中科院分区：
文献类型：
--
作者：
Khaledi,MG;Strasters,JK;Rodgers,AH;Breyer,ED
本文讨论了胶束和有机溶剂作为反相液相色谱（RPLC）中水移动的相调节剂在控制保留和选择性方面的作用。在反相高效液相色谱中，洗脱强度随有机溶剂或胶束浓度的增加而增加。在水-有机溶剂-胶束混合洗脱剂中，分离选择性随洗脱强度的增加而增强。这种选择性增强系统地发生，即峰分离单调增加的有机溶剂的体积分数添加到胶束洗脱液，并观察到大量的离子和非离子化合物与不同的官能团和两种表面活性剂（阴离子和阳离子）。对于两种测试混合物，13种氨基酸/肽和15种酚，显示在更强的混合洗脱液下观察到更好的分离和更短的分析时间。这种选择性的增强可以归因于胶束LC系统中的竞争分配平衡和/或胶束的独特特性，以区室化溶质和有机溶剂。
The role of micelles and organic solvents as the modifiers of the aqueous mobile phase In reversed-phase liquid chromatography (RPLC) In controlling retention and selectivity is discussed. Elution strength Increases In RPLC with an in-crease In organic solvent or micelle concentration. Simulta-neous enhancement of separation selectivity with elution strength In the hybrid eluents of water-organic solvent-micelles was observed. This selectivity enhancement occurs systematically, Ie peak separation increases monotonlcally with volume fraction of organic solvent added to micellar eluent, and Is observed for a large number of Ionic and non-lonlc compounds with different functional groups and for two surfactants (anionic and cationic). For two test mixtures, 13 amino acids/peptides and 15 phenols, tt Is shown that a better separation and shorter analysis time are observed at stronger hybrid eluents. This selectivity enhancement can be attributed to the competing partitioning equilibria in micellar LC systems and/or to the unique characteristics of micelles to compartmentalize solutes and organic solvents.