A role of KIT receptor signaling for proliferation and differentiation of rat stem Leydig cells in vitro
A role of KIT receptor signaling for proliferation and differentiation of rat stem Leydig cells in vitro
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KIT受体信号传导对大鼠干间质细胞体外增殖和分化的作用
DOI：
10.1016/j.mce.2017.01.023
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发表时间：
2017-03-15
影响因子：
4.1
通讯作者：
Ge, Ren-Shan
中科院分区：
文献类型：
--
作者：
Liu, Shiwen;Chen, Xiaomin;Ge, Ren-Shan
在睾丸中，KIT配体（KITL，也称为干细胞因子）由支持细胞表达，其受体（c-kit，KIT）由精原细胞和间质细胞表达。虽然KITL-KIT信号通路在精子发生中起着重要作用，但其在青春期间质细胞发育中的作用尚不清楚。在本研究中，我们研究了KITL对干Leydig细胞增殖和分化的影响。利用睾丸间质细胞缺失的睾丸生精小管体外培养系统，我们发现KITL在较高浓度（10和100 ng/ml）下增加了假定的干间质细胞的增殖活性。低浓度（1 ng/ml）的KITL通过增加类固醇生成急性调节蛋白（星星）的表达水平显着诱导斯特恩Leydig细胞分化。相反，较高浓度（100 ng/ml）的KITL通过抑制类固醇生成酶（Cyplial、Cyp 17 al和Hsd 17 b3）的表达水平来抑制Stern Leydig细胞的分化。我们用KITL培养大鼠前体Leydig细胞48 h，未发现KITL对这些细胞的增殖和雄激素产生有任何影响。总之，KITL是一种调节干Leydig细胞发育的生长因子。皇冠版权所有（C）2017由Elsevier爱尔兰有限公司发布。保留所有权利。
In the testis, KIT ligand (KITL, also called stem cell factor) is expressed by Sertoli cells and its receptor (c-kit, KIT) is expressed by spermatogonia and Leydig cells. Although KITL-KIT signaling is critical for the spermatogenesis, its roles in Leydig cell development during puberty are not clear. In the present study, we investigated effects of KITL on stem Leydig cell proliferation and differentiation. Using an in vitro culture system of seminiferous tubules from Leydig cell-depleted testis, we found that KITL increased the proliferation activity of putative stem Leydig cells at higher concentration (10 and 100 ng/ml). Low concentration (1 ng/ml) of KITL significantly induced the differentiation of stern Leydig cells via increasing the expression level of steroidogenic acute regulatory protein (Star). In contrast, higher concentration (100 ng/ml) of KITL inhibited the differentiation of stern Leydig cells via inhibiting the steroidogenic enzyme (Cyplial, Cyp17al, and Hsd17b3) expression levels. We cultured rat progenitor Leydig cells with KITL for 48 h and did not find any influence of KITL on the proliferation and androgen production of these cells. In conclusion, KITL is a growth factor that regulates the development of the stem Leydig cell. Crown Copyright (C) 2017 Published by Elsevier Ireland Ltd. All rights reserved.