Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses.
Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses.
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DOI：
10.1186/1743-422x-3-70
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发表时间：
2006-09-03
期刊：
影响因子：
4.8
通讯作者：
Cosset, Francois-Loic
中科院分区：
文献类型：
--
作者：
Szecsi, Judit;Boson, Bertrand;Johnsson, Per;Dupeyrot-Lacas, Pia;Matrosovich, Mikhail;Klenk, Hans-Dieter;Klatzmann, David;Volchkov, Viktor;Cosset, Francois-Loic
迫切需要开发针对新出现的高致病性禽流感病毒的疫苗接种新方法。在这里，我们设计的流感病毒样颗粒（流感病毒-VLP）来自逆转录病毒的核心颗粒，模拟的两个高致病性流感病毒的H7 N1或H5 N1抗原亚型的病毒表面的特性。我们证明，从野外菌株中回收病毒RNA后，可以很容易地产生编码HA，NA和M2表面蛋白的表达载体，并制备高滴度的流感病毒-VLPs。我们将这些Flu-VLP与HA、NA和M2蛋白结合，并表明它们在小鼠中诱导高滴度中和抗体。
There is an urgent need to develop novel approaches to vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered influenza viral-like particles (Flu-VLPs) derived from retroviral core particles that mimic the properties of the viral surface of two highly pathogenic influenza viruses of either H7N1 or H5N1 antigenic subtype. We demonstrate that, upon recovery of viral RNAs from a field strain, one can easily generate expression vectors that encode the HA, NA and M2 surface proteins of either virus and prepare high-titre Flu-VLPs. We characterise these Flu-VLPs incorporating the HA, NA and M2 proteins and we show that they induce high-titre neutralising antibodies in mice.