Prognostic evaluation of microRNA-210 expression in pediatric osteosarcoma
Prognostic evaluation of microRNA-210 expression in pediatric osteosarcoma
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DOI：
10.1007/s12032-013-0499-6
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发表时间：
2013-06-01
期刊：
影响因子：
3.4
通讯作者：
Wang, Zhigang
中科院分区：
文献类型：
--
作者：
Cai, Haiqing;Lin, Lijun;Wang, Zhigang
微小RNA - 210（miR - 210）在不同癌症类型中对细胞生长、血管生成和细胞凋亡的调节起着重要作用。先前对微小RNA表达谱的研究发现，miR - 210在骨肉瘤样本中显著升高。然而，其在该疾病中的作用尚未完全阐明。因此，本研究的目的是探讨miR - 210表达与骨肉瘤患者临床病理特征及预后的关系。采用实时定量逆转录聚合酶链反应分析检测92例原发性骨肉瘤患儿的癌性和非癌性骨组织中miR - 210的表达水平。与相应的非癌性骨组织相比，miR - 210在骨肉瘤组织中的表达显著增加（P < 0.001）。此外，在肿瘤体积大（P = 0.02）、术前化疗反应差（P = 0.008）和有转移（P = 0.01）的骨肉瘤组织中，miR - 210上调更为频繁。而且，miR - 210上调与总生存期（P = 0.007）和无进展生存期（P = 0.01）显著降低有关。在Cox比例风险模型中，证实其在活检样本中的表达是骨肉瘤不良生存的独立预后因素（对于总生存期：P = 0.01；对于无进展生存期：P = 0.02）。这些发现表明，miR - 210上调与儿童骨肉瘤的肿瘤侵袭性进展密切相关，并有助于对这种恶性肿瘤患者进行预后筛查。
MicroRNA-210 (miR-210) plays important roles in the regulation of cell growth, angiogenesis, and apoptosis in different cancer type. Previous study of miRNA expression profiling found that miR-210 was significantly elevated in osteosarcoma samples. However, its roles in this disease have not been fully elucidated. Thus, the aim of this study was to investigate the association of miR-210 expression with clinicopathologic features and prognosis in patients with osteosarcomas. Quantitative real-time reverse transcriptase-polymerase chain reaction analysis was performed to detect the expression level of miR-210 in cancerous and noncancerous bone tissues from 92 children treated for primary osteosarcomas. MiR-210 expression was significantly increased in osteosarcoma tissues compared with that in corresponding noncancerous bone tissues (P < 0.001). In addition, miR-210 upregulation more frequently occurred in osteosarcoma tissues with large tumor size (P = 0.02), poor response to preoperative chemotherapy (P = 0.008), and positive metastasis (P = 0.01). Moreover, miR-210 upregulation was associated with significantly decreased overall survival (P = 0.007) and progression-free survival (P = 0.01). In the Cox proportional hazard model, it was confirmed that its expression in the biopsy samples was an independent prognostic factor of unfavorable survival in osteosarcoma (for overall survival: P = 0.01; for progression-free survival: P = 0.02). These findings suggested that miR-210 upregulation showed a strong correlation with tumor aggressive progression of pediatric osteosarcoma and could help prognostic screening of patients with this malignancy.