The S haplotypes lacking SLG in the genome of Brassica rapa
The S haplotypes lacking SLG in the genome of Brassica rapa
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DOI：
10.1007/s00299-003-0598-5
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发表时间：
2003-03
影响因子：
6.2
通讯作者：
G. Suzuki;T. Kakizaki;Y. Takada;H. Shiba;S. Takayama;A. Isogai;M. Watanabe
中科院分区：
文献类型：
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作者：
G. Suzuki;T. Kakizaki;Y. Takada;H. Shiba;S. Takayama;A. Isogai;M. Watanabe
芸苔属植物的自交不亲和性（SI）是由S位点基因调控的，它能区分自交和非自交的花粉。在大多数单倍型中，高度多态性的S位点糖蛋白（SLG）基因与SI决定簇、S受体激酶（SRK）和SP 11基因紧密连锁，尽管SLG在SI中的确切功能尚未阐明。本研究对具有正常SI表型的芸苔属油菜的S32、S33和S36单倍型进行了DNA凝胶印迹分析，结果表明它们的基因组中可能不存在SLG。RNA凝胶印迹分析表明，SRK基因可能存在于柱头中。这三种单体型是研究SI反应的分子机制的有用资源。
Self-incompatibility (SI) discriminating self- and non-self pollen is regulated byS-locus genes inBrassica. In most of theShaplotypes, a highly polymorphicS-locus glycoprotein (SLG) gene is tightly linked to genes for the SI determinants,S-receptor kinase (SRK) andSP11, although the precise function of SLG in SI has not been clarified. In the present study, we performed DNA gel blot analysis forS32,S33, andS36haplotypes ofBrassica rapashowing normal SI phenotypes and concluded that there might be noSLGin their genome. RNA gel blot analysis of theSLG-lessShaplotypes indicated the possible existence ofeSRKtranscripts in the stigma. These threeShaplotypes are useful resources to discern the molecular mechanism of the SI reaction withoutSLG.