Total hydroperoxide and biological antioxidant potentials in a neonatal sepsis model
Total hydroperoxide and biological antioxidant potentials in a neonatal sepsis model
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DOI：
10.1203/01.pdr.0000245911.79943.8a
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发表时间：
2006-12-01
影响因子：
3.6
通讯作者：
Togari, Hajime
中科院分区：
文献类型：
--
作者：
Kakita, Hiroki;Hussein, Mohamed H.;Togari, Hajime
氧化剂/抗氧化剂失衡在感染性休克中发挥着重要作用。本研究探讨了新生儿败血症模型中循环氧化成分的变化。受试者为14头新生混合品系仔猪，随机分为两组：盲肠结扎穿孔（CLP）模型组（n = 7）和假手术组（n = 7）。在CLP前和CLP后1、3和6 h采集血液样本，用于总氢过氧化物（TH）、生物抗氧化潜力（BAP）、肿瘤坏死因子（TNF）α、白细胞介素（IL）-6和IL-10。CLP后1h，CLP组TH和BAP水平显著高于Sham组。CLP组TH逐渐下降，至CLP后6 h降至基线水平，BAP仍升高。CLP后1 h血清TH与BAP、CLP后1 h血清TH与TNF-α、CLP后6 h血清BAP与IL-6呈线性相关。阐明了新生儿败血症模型中循环氧化和炎症状态组分的变化及其相关性。这是第一个研究表明，在脓毒症和脓毒性休克的氧化剂/抗氧化剂失衡的存在下，在疾病过程中的变化。
Oxidant/antioxidant imbalance plays an important role in septic shock. The present study examined changes in circulating oxidative components in a neonatal sepsis model. Subjects were 14 newborn mixed-strain piglets randomly divided into two groups: a cecal ligation and perforation (CLP) model (n = 7) and sham (n = 7). Blood samples for total hydroperoxide (TH), biological antioxidant potential (BAP), tumor necrosis factor (TNF) alpha, interleukin (IL)-6, and IL-10 were collected pre-CLP and at 1, 3, and 6 h post-CLP. TH and BAP levels at 1 h post-CLP were significantly higher in the CLP group than in the sham group. In the CLP group, TH decreased gradually and reached baseline levels by 6 h post-CLP, while BAP remained elevated. Linear correlations were identified between serum TH and BAP at 1 h post-CLP, serum TH and TNF-alpha at 1 h post-CLP, and BAP and IL-6 at 6 h post-CLP. Changes in and correlations between circulating oxidative and inflammatory state components in a neonatal sepsis model were clarified. This is the first study to reveal that the presence of oxidant/antioxidant imbalance in sepsis and septic shock changes during the disease course.