Entamoeba histolytica:: Cyst-like structures in vitro induction
Entamoeba histolytica:: Cyst-like structures in vitro induction
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DOI：
10.1016/j.exppara.2007.11.002
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发表时间：
2008-04-01
影响因子：
2.1
通讯作者：
Morales-Vallarta, M. R.
中科院分区：
文献类型：
--
作者：
Barron-Gonzalez, M. P.;Villarreal-Trevino, L.;Morales-Vallarta, M. R.
溶组织内阿米巴包囊是阿米巴感染的主要原因。然而，没有无菌的体外系统存在，促进大规模包囊的研究这一过程中的人类感染的寄生虫。用TYI-S-33培养基与肠杆菌大肠杆菌和粪肠球菌条件培养基、高CO2张力和组胺联合诱导溶组织大肠杆菌的囊状结构。囊样结构显示出典型溶组织埃立克体囊的相同特征：聚集，对0.15%肌氨酸10分钟的抗性，当用10% calcofluor M2 r染色时在UV光下的高荧光信号，并且表面拓扑结构显示出褶皱壁。此外，这些结构是多核的，染色质浓缩附着在核膜上，在细胞质中含有大空泡和核糖核蛋白螺旋，并且还存在薄的细胞壁。所有特征均与典型的溶组织埃立克体囊肿相同。(c)2007年爱思唯尔公司All rights reserved.
The cyst of Entamoeba histolytica is responsible for amebiasis infection. However, no axenic in vitro system exists that promotes mass encystation for studying this process of this human-infecting parasite. Cyst-like structures of E histolytica obtained in this work were induced using TYI-S-33 media in combination with enterobacterias Escherichia coli and Enterococcus faecalis conditioned media, high CO2 tension and histamine. Cyst-like structures showed the same characteristics of a typical E histolytica cyst: aggregation, resistance to 0.15% sarcosyl for 10 min, high signal of fluorescence under UV light when stained with 10% calcofluor M2r and the surface topology showed a wrinkled wall. In addition these structures are multinucleated with condensed chromatin attached to nuclear membrane, contain big vacuoles and ribonucleoproteic helices in the cytoplasm and also present a thin cell wall. Last all characteristics are all the same as a typical of E histolytica cyst. (c) 2007 Elsevier Inc. All rights reserved.