Salivaricin A2 and the novel lantibiotic salivaricin B are encoded at adjacent loci on a 190-kilobase transmissible megaplasmid in the oral probiotic strain Streptococcus salivarius K12
Salivaricin A2 and the novel lantibiotic salivaricin B are encoded at adjacent loci on a 190-kilobase transmissible megaplasmid in the oral probiotic strain Streptococcus salivarius K12
复制标题
DOI：
10.1128/aem.02265-06
复制
发表时间：
2007-02-01
影响因子：
4.4
通讯作者：
Tagg, John R.
中科院分区：
文献类型：
--
作者：
Hyink, Otto;Wescombe, Philip A.;Tagg, John R.
商业益生菌唾液链球菌菌株K12是这些S.在链球菌细菌素生产的标准化试验中具有最强抑制作用的唾液链球菌菌株，并且已经显示出产生2，368-Da唾液菌素A2（SalA 2）和2，740-Da唾液菌素B（Sbo B）硫醚抗生素。先前未表征的SboB属于lantibiotic细菌素的类型All类，并且由八个基因簇编码。菌株K12中编码SalA 2和SboB的遗传基因座已被充分表征，并定位于pSsal-K12（190-kb的大质粒）上几乎相邻的位点。对61株具有较强抑菌活性的S. 19例（31%）sboB结构基因阳性。这19个菌株中除一个菌株（NR菌株）外的所有菌株也对salA 2呈阳性，并且在这些双重阳性的每一个病例中，两个基因座相隔不到10 kb。这是首次报道一株链霉菌产生两种不同的硫醚抗生素。
The commercial probiotic Streptococcus salivarius strain K12 is the prototype of those S. salivarius strains that are the most strongly inhibitory in a standardized test of streptococcal bacteriocin production and has been shown to produce the 2,368-Da salivaricin A2 (SalA2) and the 2,740-Da salivaricin B (SboB) lantibiotics. The previously uncharacterized SboB belongs to the type All class of lantibiotic bacteriocins and is encoded by an eight-gene cluster. The genetic loci encoding SalA2 and SboB in strain K12 have been fully characterized and are localized to nearly adjacent sites on pSsal-K12, a 190-kb megaplasmid. Of 61 strongly inhibitory strains of S. salivarius, 19 (31%) were positive for the sboB structural gene. All but one (strain NR) of these 19 strains were also positive for salA2, and in each of these cases of double positivity, the two loci were separated by fewer than 10 kb. This is the first report of a single streptococcus strain producing two distinct lantibiotics.