Model for detection of immobilized superparamagnetic nanosphere assay labels using giant magnetoresistive sensors
Model for detection of immobilized superparamagnetic nanosphere assay labels using giant magnetoresistive sensors
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DOI：
10.1116/1.582476
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发表时间：
2000-07
期刊：
影响因子：
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通讯作者：
M. Tondra;M. Porter;R. J. Lipert
中科院分区：
文献类型：
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作者：
M. Tondra;M. Porter;R. J. Lipert
市售的超顺磁性纳米球通常用于广泛的生物应用，特别是在磁辅助分离中。一种新的和潜在的重要技术涉及使用这些颗粒作为标记在磁阻测定应用。在这些测定中，磁珠标记与荧光标记一样使用，不同之处在于珠用磁场而不是用光子激发和检测。这种技术的一个主要优点是，用于激励和检测的装置很容易集成在硅电路上。对该技术的初步研究证明了其基本可行性，并预测灵敏度优于10 - 12摩尔[Baselt等人，Biosensors Bioelectronic 13，731（1998）]。在这篇文章中，我们研究的理论信号噪声比，这种类型的分析的特殊情况下，一个单一的磁珠被检测到一个单一的巨磁阻（GMR）检测器。假设实验观察和推理…
Commercially available superparamagnetic nanospheres are commonly used in a wide range of biological applications, particularly in magnetically assisted separations. A new and potentially significant technology involves the use of these particles as labels in magnetoresistive assay applications. In these assays, magnetic bead labels are used like fluorescent labels except that the beads are excited and detected with magnetic fields rather than with photons. A major advantage of this technique is that the means for excitation and detection are easily integrable on a silicon circuit. A preliminary study of this technique demonstrated its basic feasibility, and projected a sensitivity of better than 10−12 molar [Baselt et al., Biosensors Bioelectronic 13, 731 (1998)]. In this article we examine the theoretical signal to noise ratio of this type of assay for the special case of a single magnetic bead being detected by a single giant magnetoresistive (GMR) detector. Assuming experimentally observed and reasona...