Colorimetric detection of urea, urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles
Colorimetric detection of urea, urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles
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基于金纳米粒子的类过氧化物酶活性的尿素、尿素酶和尿素酶抑制剂的比色检测
DOI：
10.1016/j.aca.2016.02.008
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发表时间：
2016-04-07
影响因子：
6.2
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Deng, Hao-Hua;Hong, Guo-Lin;Chen, Wei
本文首次报道了金纳米粒子催化的3，3 '，5，5'-四甲基联苯胺-H2 O2体系可以作为一种超灵敏的比色pH指示剂。金纳米粒子作为催化剂，模仿辣根过氧化物酶的功能。在pH 6.40-6.60范围内，黄色产物在450 nm处的吸光度呈线性关系。基于这一特性，我们构建了一种新的传感平台，用于尿素，脲酶和脲酶抑制剂的测定。尿素和脲酶的检测限分别为5 mM和1.8 U/L。乙酰异羟肟酸的半最大抑制IC_（50）为0.05mM，对人尿中尿素和土壤中脲酶的测定结果满意。(C)© 2016 Elsevier B. V.版权所有。
Herein, we reported for the first time that gold nanoparticles-catalyzed 3,3',5,5'-tetramethylbenzidine-H2O2 system can serve as an ultrasensitive colorimetric pH indicator. Gold nanoparticles acted as a catalyst and imitated the function of horseradish peroxidase. The absorbance at 450 nm of the yellow-color product in the catalytic reaction exhibited a linear fashion over the pH range of 6.40-6.60. On the basis of this property, we constructed a novel sensing platform for the determination of urea, urease, and urease inhibitor. The limit of detection for urea and urease was 5 mM and 1.8 U/L, respectively. The half-maximal inhibition value IC50 of acetohydroxamic acid was found to be 0.05 mM. Urea in human urine and urease in soil were detected with satisfied results. (C) 2016 Elsevier B.V. All rights reserved.