Sexually dimorphic traits in the silkworm, Bombyx mori, are regulated by doublesex
Sexually dimorphic traits in the silkworm, Bombyx mori, are regulated by doublesex
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家蚕（Bombyx mori）的性二态性状受双性调控
DOI：
10.1016/j.ibmb.2016.11.005
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发表时间：
2017
影响因子：
3.8
通讯作者：
Yongping Huang
中科院分区：
文献类型：
--
作者：
Jun Xu;Shuai Zhan;Shuqing Chen;Baosheng Zeng;Zhiqian Li;Anthony A James;Anjiang Tan;Yongping Huang
昆虫中的DM结构域基因doublemex（dsx），线虫中的雄性异常3（male anomalous 3，mAb-3）和哺乳动物中的DM结构域基因Dmrt 1（doublemexandmab-3-related transcription factor 1，Dmrt 1）是性别决定途径的下游调控因子，控制两性异形发育。尽管dsx的功能重要性和其在昆虫不育技术（SIT）的潜在应用，它控制性二型性状和随后的发育基因网络在昆虫的机制知之甚少。系统发育分析表明，昆虫xgenes具有性别特异性的选择性剪接异构体，而其他类群没有。我们利用基因组编辑和转基因技术在鳞翅目模式昆虫家蚕的体细胞组织中诱导dsin的雄性特异性亚型（dsxM）或共同区域（dsxC）的突变。基因功能的破坏产生雄性特异性的性二态缺陷或间性表型;这些结果不同于在其他昆虫中观察到的结果，包括果蝇。我们的数据提供了见解的分歧昆虫性别决定途径相关的最保守的下游组件dsx。
The DM domain genes,doublesex(dsx) in insects, or their structural homologs,male abnormal 3(mab-3) in nematodes andDmrt1(doublesexandmab-3-related transcription factor 1) in mammals, are downstream regulators of the sex determination pathway that control sexually dimorphic development. Despite the functional importance ofdsxand its potential applications in sterile insect technologies (SITs), the mechanisms by which it controls sexually dimorphic traits and the subsequent developmental gene networks in insects are poorly understood. Phylogenetic analyses indicate that insectdsxgenes have sex-specific alternative splicing isoforms, whereas other taxa do not. We exploited genome editing and transgenesis technologies to induce mutations in either the male-specific isoform (dsxM) or common region (dsxC) ofdsxin the somatic tissues of the lepidopteran model insectBombyx mori. Disruptions of gene function produced either male-specific sexually-dimorphic defects or intersexual phenotypes; these results differ from those observed in other insects, includingDrosophila melanogaster. Our data provide insights into the divergence of the insect sex determination pathways related to the most conserved downstream componentdsx.