Rapid neurotransmitter uncaging in spatially defined patterns
Rapid neurotransmitter uncaging in spatially defined patterns
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DOI：
10.1038/nmeth793
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发表时间：
2005-11-01
期刊：
影响因子：
48
通讯作者：
Wang, SSH
中科院分区：
文献类型：
--
作者：
Shoham, S;O'Connor, DH;Wang, SSH
光敏“笼”分子提供了一种快速和非侵入性地操纵生化信号与亚微米空间分辨率的手段。在这里，我们描述了一种新的光学系统，用于以任意模式快速解开，以模拟复杂的神经活动。该系统使用TeO2声光偏转器来快速引导紫外线光束，每秒可以释放超过20，000个位置。释放光束投射到双光子显微镜的焦平面上，使我们能够将图案化的释放与成像和电生理学结合起来。通过光解大脑切片中的笼状神经递质，我们可以为树突整合产生精确、复杂的活动模式。该方法也可以用于同时激活许多突触前神经元。图案化解除包裹为神经元回路和其他生物系统中信号整合和可塑性的研究开辟了新的前景。
Light-sensitive 'caged' molecules provide a means of rapidly and noninvasively manipulating biochemical signals with submicron spatial resolution. Here we describe a new optical system for rapid uncaging in arbitrary patterns to emulate complex neural activity. This system uses TeO2 acousto-optical deflectors to steer an ultraviolet beam rapidly and can uncage at over 20,000 locations per second. The uncaging beam is projected into the focal plane of a two-photon microscope, allowing us to combine patterned uncaging with imaging and electrophysiology. By photolyzing caged neurotransmitter in brain slices we can generate precise, complex activity patterns for dendritic integration. The method can also be used to activate many presynaptic neurons at once. Patterned uncaging opens new vistas in the study of signal integration and plasticity in neuronal circuits and other biological systems.