The O-antigen structure of bacterium Comamonas aquatica CJG
The O-antigen structure of bacterium Comamonas aquatica CJG
复制标题
水生丛毛单胞菌 CJG 细菌的 O 抗原结构
DOI：
10.1099/mic.0.000551
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发表时间：
2017
期刊：
影响因子：
1.5
通讯作者：
Aidong Han
中科院分区：
文献类型：
--
作者：
Xiqian Wang;Anna N. Kondakova;Yutong Zhu;Yuriy A. Knirel;Aidong Han
丛毛单胞菌属是一组能够降解各种环境废物的细菌。水生丛毛单胞菌（Comamonas aquatica CJG）aquatica）能够吸收人血清中的低密度脂蛋白，但不能吸收人血清中的高密度脂蛋白。使用1H和13 C NMR光谱，我们发现该细菌的O-多糖（O-抗原）由D-葡萄糖和2-O-乙酰基-L-鼠李糖的二糖重复（O-单元）组成，这是粘质沙雷氏菌O 6所共有的。C. O抗原基因簇。aquatica位于coaX和tnp 4基因之间，含有鼠李糖合成基因、糖基和乙酰基转移酶基因以及ATP结合盒转运蛋白基因，因此与此处确定的O-抗原结构一致。
Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste. Comamonas aquatica CJG (C. aquatica) in this genus is able to absorb low-density lipoprotein but not high-density lipoprotein of human serum. Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of D-glucose and 2-O-acetyl-L-rhamnose, which is shared by Serratia marcescens O6. The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes,.glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.