Demonstration of scrapie strain diversity in infected PC12 cells.
Demonstration of scrapie strain diversity in infected PC12 cells.
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证明受感染的 PC12 细胞中痒病菌株的多样性。
DOI：
10.1099/0022-1317-73-11-3027
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Carp,RI
中科院分区：
文献类型：
--
作者：
Rubenstein,R;Deng,H;Race,RE;Ju,W;Scalici,CL;Papini,MC;Kascsak,RJ;Carp,RI
检查了瘙痒病株在神经生长因子诱导的分化 PC12 细胞培养系统中的复制。小鼠来源的病原体之间的复制差异得到了证实，139A 瘙痒病菌株产生的感染性水平比 ME7 瘙痒病菌株高 100 至 1000 倍。在感染仓鼠来源的 263K 或大鼠来源的 139R 瘙痒病菌株的 PC12 细胞中未检测到复制。对受感染 PC12 细胞中神经递质的研究表明，肾上腺素能途径未改变，但胆碱能途径发生了改变。此外，改变的程度与痒病菌株复制的水平相关。将 ME7 感染的 PC12 细胞与 Chandler 试剂感染的小鼠神经母细胞瘤细胞的感染滴度和酶学变化进行比较表明，表现出低感染滴度的培养物中神经递质水平的显着变化还涉及除菌株复制之外的因素。讨论了 PC12 细胞中瘙痒病菌株复制范围的变化与物种屏障、细胞靶向、遗传易感性和物种菌株特异性的关系。这些研究进一步强调了 PC12 细胞模型系统在检查瘙痒病菌株与宿主细胞相互作用方面的价值，此外还支持瘙痒病菌株之间变异的概念。
Scrapie strain replication in the nerve growth factor-induced, differentiated PC12 cell culture system was examined. Differences in replication between mouse-derived agents were demonstrated, with the 139A scrapie strain yielding 100- to 1000-fold higher levels of infectivity than the ME7 scrapie strain. Replication was not detected in PC12 cells infected with either the hamster-derived 263K or rat-derived 139R scrapie strains. Studies on the neurotransmitters in infected PC12 cells demonstrated that the adrenergic pathway was unchanged but the cholinergic pathway was altered. Furthermore, the degree of alteration correlated with the level of scrapie strain replication. Comparison of infectivity titres and enzymatic changes in ME7-infected PC12 cells with those in Chandler agent-infected mouse neuroblastoma cells suggests that the significant changes in neurotransmitter levels in cultures exhibiting low titres of infectivity involve factors in addition to strain replication. The variation in the range of scrapie strain replication in PC12 cells is discussed in relationship to species barrier, cell targeting, genetic susceptibility and species strain specificity. These studies further emphasize the value of the PC12 cell model system in examining the scrapie strain-host cell interaction and in addition support the concept of variation among scrapie strains.