Localization of TNF-α and Macrophages in the Periodontal Ligament during Orthodontic Tooth Movement
Localization of TNF-α and Macrophages in the Periodontal Ligament during Orthodontic Tooth Movement
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正畸牙齿移动过程中 TNF-α 和巨噬细胞在牙周膜中的定位
DOI：
10.5466/ijoms.11.182
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
K. Kasai
中科院分区：
文献类型：
--
作者：
Mari Funakoshi;M. Yamaguchi;S. Fujita;K. Kasai
牙周组织在施加机械力后的重塑构成了临床牙周病学的基础，各种免疫调节分子参与了这一过程。本研究观察了大鼠实验性牙齿移动过程中牙周膜（PDL）中肿瘤坏死因子-α（TNF-α）和巨噬细胞的分布。15只6周龄雄性Wistar大鼠，施加10 g的正畸力，诱导上颌第一磨牙向近中倾斜。实验性牙齿移动完成了七天。我们用免疫组织化学方法测定了正畸牙齿移动过程中牙周膜中TNF-α和RM-4（一种识别巨噬细胞的特异性抗体）的定位。10 g压力组牙周膜成纤维细胞中TNF-α和RM-4的免疫反应阳性。移动后第1天，TNF-α和RM-4的免疫反应较弱。第3、5天，成纤维细胞部分细胞核内TNF-α和RM-4的阳性反应较第1天明显增多。此外，这些阳性反应在7天后减少。因此，参与TNF-α表达的RM-4（+）细胞可能在牙周膜的初始反应和正畸牙移动过程中骨吸收的诱导中起重要作用。
Remodelling of the periodontium after application of mechanical forces constitutes the basis of clinical orthodontics, and various immunoregulatory molecules are involved in this process. The present study focused on the localizations of the tumor necrosis factor-α (TNF-α)and macrophages in periodontal ligament(PDL)during experimental tooth movement in rats. A total of 15 male 6-weeks-old Wistar rats were subjected to an orthodontic force of 10g to induce a mesially tipping movement of the upper first molars. Experimental tooth movement was accomplished for seven days. We determined the localization of TNF-α and RM-4(an antibody specific for identification of macrophages)in the PDL during orthodontic tooth movement using immunohistochemistry. Immunoreactivity for TNF-α and RM-4 was detected in PDL fibroblasts in the compressive side by the orthodontic force of 10g. On the first day after tooth movement, the immunoreactivity of TNF-α and RM-4 was weak. On the third and fifth days, more TNF-α and RM-4 positive reactions in some nucleuses of fibroblasts were recognized than on the first day. Furthermore, these positive reactions were decreased after seven days. Therefore, RM-4 (+)cells involved in the expression of TNF-α may play an important role in the initial reaction of the PDL and in the induction of the osteoclastic bone resorption during orthodontic tooth movement.
影响因子：
4.4
作者：
F. Dewhirst;P. Stashenko;J. Mole;T. Tsurumachi
通讯作者：
F. Dewhirst;P. Stashenko;J. Mole;T. Tsurumachi
DOI：
10.1016/0030-4220(94)90044-2
发表时间：
1994-10-01
影响因子：
--
作者：
STASHENKO, P;WANG, CY;YU, SM
通讯作者：
YU, SM