Elexacaftor-tezacaftor-ivacaftor decreases pseudomonas abundance in the sinonasal microbiome in cystic fibrosis.
Elexacaftor-tezacaftor-ivacaftor decreases pseudomonas abundance in the sinonasal microbiome in cystic fibrosis.
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Elexacaftor-tezacaftor-ivacaftor 降低囊性纤维化鼻窦微生物组中假单胞菌的丰度。
DOI：
10.1002/alr.23288
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发表时间：
2024
影响因子：
6.4
通讯作者：
Cooper,VaughnS
中科院分区：
文献类型：
--
作者：
Zemke,AnnaC;Hilliam,Yasmin;Stapleton,AmandaL;Kimple,AdamJ;Goralski,JenniferL;Shaffer,AmberD;Pilewski,JosephM;Senior,BrentA;Lee,StellaE;Cooper,VaughnS
慢性鼻-鼻窦炎(CRS)在囊性纤维化(CF)患者中很常见，以慢性炎症和感染发作为特征，对生活质量产生负面影响。多项研究表明，elexaftor-tezacaftor-iVacaftor(ETI)可改善CF-CRS的症状和检查结果。本研究旨在探讨ETI对鼻腔内微生物区系的影响。方法在ETI前、后在鼻内窥镜下采集鼻腔标本。对样品进行16S扩增序列测定，序列用QIIME2流水线处理，然后用素食R包进行分析。结果ETI前后共有29份个体基线样本和23对样本。基线时，队列样本以葡萄球菌为主，阿尔法多样性低于公布的无鼻窦疾病个体的参考组。根据Shannon指数、观察到的丰富度和Faith的系统发育多样性指数，曾接受过鼻窦手术的个体具有较低的α多样性。变应性鼻炎患者和非变应性鼻炎患者的β多样性不同，变应性鼻炎患者的葡萄球菌丰度较高。在ETI的中位数9个月后，α或β多样性没有变化。利用ETI，假单胞菌属和含有伯克霍尔氏菌的属在基线上减少了，qPCR检测到假单胞菌丰度随着处理的减少。葡萄球菌、棒状杆菌和链球菌的核心菌群成员没有变化，而Moraxellas随着ETI的增加而增加。结论ETI治疗后，鼻腔内假单胞菌的丰度降低。
BackgroundChronic rhinosinusitis (CRS) is common in individuals with cystic fibrosis (CF) and is marked by chronic inflammation and episodes of infection that negatively impact quality of life. Several studies have shown that elexacaftor–tezacaftor–ivacaftor (ETI) improves symptoms and examination findings in CF‐CRS. The current study determines the effect of ETI on the sinonasal microbiota in CF.MethodsSinonasal samples were collected under endoscopic visualization before and after starting ETI. Samples were subjected to 16S amplicon sequencing and sequences were processed with the QIIME2 pipeline with subsequent analysis using the vegan R‐package.ResultsTwenty‐nine individual baseline samples and 23 sample pairs pre‐/post‐ETI were available. At baseline, the cohort had samples dominated byStaphylococcus, and alpha diversity was lower than that of a published reference set of individuals without sinonasal disease. Individuals with prior sinus surgery had lower alpha diversity as measured by Shannon Index, Observed Richness, and Faith's phylogenetic diversity Index. Beta diversity differed between individuals with and without allergic rhinitis, with higherStaphylococcusabundance in those with allergic rhinitis. No change in alpha or beta diversity was seen after a median of 9 months on ETI. With ETI, thePseudomonasgenus and the genus containingBurkholderiadecreased in samples containing these taxa at baseline.Pseudomonasabundance decreased with treatment as measured by qPCR. Core sinonasal microbiome membersStaphylococcus,Corynebacterium, andStreptococcuswere unchanged, whileMoraxellaincreased with ETI.ConclusionsTreatment with ETI leads to a reduction inPseudomonasabundance within the sinonasal microbiome of individuals withPseudomonasat baseline.
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.6
作者：
Beaume M;Köhler T;Greub G;Manuel O;Aubert JD;Baerlocher L;Farinelli L;Buckling A;van Delden C;Swiss Transplant Cohort Study
通讯作者：
Swiss Transplant Cohort Study
影响因子：
10
作者：
Mainz, J. G.;Naehrlich, L.;Tuemmler, B.
通讯作者：
Tuemmler, B.