Immunohistochemical analysis of cholecystokinin A receptor distribution in the rat pancreas
Immunohistochemical analysis of cholecystokinin A receptor distribution in the rat pancreas
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DOI：
10.1016/j.regpep.2004.08.033
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发表时间：
2005-03-15
影响因子：
--
通讯作者：
Shioda, S
中科院分区：
文献类型：
--
作者：
Kageyama, H;Kita, T;Shioda, S
胆囊收缩素（CCK）通过与CCK- a受体（CCK- ar）结合，在胰腺酶分泌调控中发挥重要作用。虽然已知CCK-AR在大鼠胰岛B细胞中表达，但CCK-AR在大鼠胰腺A细胞和D细胞中的定位仍然知之甚少。本研究的目的是利用抗CCK-AR和胰高血糖素抗体的双重免疫荧光法确定CCK-AR在大鼠胰岛中的定位。胰岛素和生长抑素的原位杂交检测其转录物。cck - ar样免疫反应细胞与胰高血糖素样免疫反应细胞和胰岛素样免疫反应细胞重叠，但与生长抑素样免疫反应细胞不重叠。利用cRNA探针对CCK-AR进行原位杂交研究发现，CCK-AR mRNA在死亡胰岛的中心和周围表达。除此之外。CCK-原位杂交后，用抗胰高血糖素抗体进行免疫荧光染色。AR rna探针，以鉴定表达CCK-AR mRNA的细胞。CCK-AR mRNA的分布模式与胰高血糖素样免疫反应细胞的分布模式几乎相同。这些结果清楚地表明，CCK- ar不仅存在于大鼠胰腺的B细胞中，也存在于A细胞中，表明CCK至少部分通过CCK- ar调节胰岛素和胰高血糖素的分泌。(C) 2004 Elsevier B.V.版权所有
Cholecystokinin (CCK) plays a major role in the regulation of pancreatic enzyme secretion based on its binding to the CCK-A receptor (CCK-AR). While CCK-AR is known to be expressed in rat islet B cells, the localization of CCK-AR in rat pancreatic A and D cells remains poorly understood. The aim of this study was to identify the localization of CCK-AR in rat pancreatic islets by means of double immunofluorescence straining with antibodies against CCK-AR, glucagon. insulin and somatostatin and with in situ hybridization to detect 9 its transcript. CCK-AR-like immunoreactive cells were found to overlap both with glucagon-like immunoreactive cells and insulin-like immunoreactive cells but not with somatostatin-like immunoreactive cells. An in situ hybridization study using a cRNA probe for CCK-AR revealed that CCK-AR mRNA was expressed in the center and periphery of die pancreatic islets. Further to this. immunofluoresence staining using anti-glucagon antibody was carried out after in situ hybridization using the CCK-.AR cRNA probe in order to identify CCK-AR mRNA expressing cells. CCK-AR mRNA exhibited a distribution pattern almost identical to that of glucagon-like immunoreactive cells. These results show clearly that CCK-AR exists not only in B but also in A cells of the rat pancreas, suggesting that CCK regulates the secretion of insulin and glucagon at least partly via CCK-AR. (C) 2004 Elsevier B.V. All rights reserved.