3′-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein
3′-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein
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DOI：
10.1016/j.febslet.2009.06.045
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发表时间：
2009-07-21
期刊：
影响因子：
3.5
通讯作者：
Yanagawa, Hiroshi
中科院分区：
文献类型：
--
作者：
Horisawa, Kenichi;Imai, Takao;Yanagawa, Hiroshi
Musashi1(Msi1)是一种在神经干细胞中高表达的RNA结合蛋白，被认为是一种干性因子。MSI1的一个已知功能是对特定结合的mRNAs的翻译抑制。虽然已经报道了MSI1的基本机制和一些靶向RNA，但为了了解MSI1的整合功能，有必要对相互作用进行进一步的研究。通过mRNA展示技术的筛选，我们发现DCX(Doublecortin，DCX)是Msi1的体外特异结合靶点。我们证实，在Neuro2A细胞中，Msi1抑制了与DCX选择的3‘-非翻译区片段相连的荧光素酶报告基因的翻译。(C)2009年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem cells, and is considered to be a stemness factor. A known function of Msi1 is translational repression of specifically bound mRNAs. Although the basic mechanism and some target RNAs have been reported, further survey of interactors is necessary to understand the integrated function of Msi1. By screening using an mRNA display technique, we found that doublecortin (dcx) mRNA is a specific binding target of Msi1 in vitro. We confirmed that Msi1 repressed translation of a luciferase reporter gene linked to the selected 3'-untranslated region fragment of dcx in Neuro2A cells. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.