Identification and comparative analysis of three novel C-type lectins from the silkworm with functional implications in pathogen recognition
Identification and comparative analysis of three novel C-type lectins from the silkworm with functional implications in pathogen recognition
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DOI：
10.1016/j.dci.2009.01.005
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Sato, Ryoichi
中科院分区：
文献类型：
--
作者：
Takase, Hinako;Watanabe, Ayako;Sato, Ryoichi
C型凝集素可以作为先天免疫中的模式识别受体（PRR）。以前，我们从家蚕（家蚕），BmLBP和BmMBP，作为PRRs的两个C型凝集素。在本研究中，我们确定了三个同源的这些凝集素通过搜索家蚕基因组数据库。这些小说B.将人低表达凝集素命名为BmLEL-1、BmLEL-2和BmLEL-3。虽然蛋白质印迹分析未能检测到血浆中的BmLEL-1，-2，或-3，幼虫血浆与各种微生物的亲和沉淀显示，BmLEL-1和-2结合的革兰氏阴性菌的粗糙和光滑的菌株，分别。BmLEL-1、BmLEL-2和BmLEL-3在睾丸和卵巢中均有表达，其中BmLEL-2在细菌感染后表达上调。这些结果表明，新的C型凝集素可能发挥作用，在这些组织中的先天免疫PRRs。在这里，我们讨论的角色和成员的C型凝集素作为主要的PRR在B。桑细胞免疫(C)2009爱思唯尔有限公司版权所有。
C-type lectins can act as pattern recognition receptors (PRRs) in innate immunity. Previously, we identified two C-type lectins from silkworm (Bombyx mori), BmLBP and BmMBP, as PRRs. In the present study, we identified three homologs of these lectins by searching the silkworm genome database. These novel B. man low-expression lectins were designated BmLEL-1, BmLEL-2, and BmLEL-3. Although Western-blot analysis failed to detect BmLEL-1, -2, or -3 in plasma, affinity precipitation of larval plasma with various microorganisms revealed that BmLEL-1 and -2 bind to rough and smooth strains of Gram-negative bacteria, respectively. BmLEL-1, -2, and -3 were found to be expressed in testis and ovary, where BmLEL-2 expression was up-regulated after bacteria infection. These results indicate that the novel C-type lectins might play a role in the innate immunity in these tissues as PRRs. Here, we discuss the roles and members of the C-type lectins as primary PRRs in B. mori cellular immunity. (C) 2009 Elsevier Ltd. All rights reserved.