Variable expression of cellular retinol- and cellular retinoic acid-binding proteins in the rat uterus and ovary during the estrous cycle.
Variable expression of cellular retinol- and cellular retinoic acid-binding proteins in the rat uterus and ovary during the estrous cycle.
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DOI：
10.1095/biolreprod56.1.125
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发表时间：
1997
影响因子：
3.6
通讯作者：
S. A. Wardlaw;R. A. Bucco;W. Zheng;D. Ong
中科院分区：
文献类型：
--
作者：
S. A. Wardlaw;R. A. Bucco;W. Zheng;D. Ong
卵巢和子宫从正常成年雌性大鼠在已知阶段的发情周期进行了分析的存在下，细胞视黄醇结合蛋白（CRBP）和两种类型的细胞视黄酸结合蛋白（CRABP和CRABP II）。子宫的北方和Western印迹分析显示CRBP在动情间期和动情前期的峰值，而CRABP II在动情期急剧达到峰值。免疫组化研究表明，CRABP II定位于腔上皮，而CRBP和CRABP只观察到在平滑肌层的子宫。在卵巢中，CRABP未检测到，而CRBP水平在整个周期中保持相对恒定，CRABP II在动情后期略有峰值。CRBP在卵巢中定位于卵母细胞、颗粒细胞附近和基质的某些区域。CRABP II主要存在于成熟卵泡和早期黄体的颗粒细胞以及间质的某些区域。这些结果表明，需要进一步的研究，以评估视黄醇及其代谢产物在正常子宫功能和卵巢卵泡发育的作用。
Ovaries and uteri from normal adult female rats at known stages of the estrous cycle were analyzed for the presence of cellular retinol-binding protein (CRBP) and both types of cellular retinoic acid-binding protein (CRABP and CRABP II). Northern and Western blot analysis of the uteri revealed a peak of CRBP during diestrus and a peak of CRABP during proestrus, whereas CRABP II peaked sharply during estrus. Immunohistochemical studies showed CRABP II localized to the luminal epithelium, while both CRBP and CRABP were observed only in the smooth muscle layers of the uterus. In the ovary, CRABP was not detected, while CRBP levels remained relatively constant throughout the cycle and CRABP II peaked slightly during metestrus. CRBP in the ovary was localized to the oocytes, nearby granulosa cells, and some regions of stroma. CRABP II was found predominantly in the granulosa cells of mature follicles and early corpora lutea, as well as some regions of the stroma. These results suggest a need for further studies to assess the role of retinol and its metabolites in normal uterine function and ovarian follicular development.