DIFFERENT CELLULAR-DISTRIBUTION OF THIOREDOXIN AND SUBUNIT M1 OF RIBONUCLEOTIDE REDUCTASE IN RAT-TISSUES
DIFFERENT CELLULAR-DISTRIBUTION OF THIOREDOXIN AND SUBUNIT M1 OF RIBONUCLEOTIDE REDUCTASE IN RAT-TISSUES
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DOI：
10.1016/0014-4827(86)90067-4
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发表时间：
1986-04-01
影响因子：
3.7
通讯作者：
HOLMGREN, A
中科院分区：
文献类型：
--
作者：
HANSSON, HA;ROZELL, B;HOLMGREN, A
用免疫组织化学方法研究了硫氧还蛋白和核苷酸还原酶蛋白M1在成年大鼠组织中的细胞分布。比较了有丝分裂或减数分裂细胞分裂频率高或低的组织。硫氧还蛋白在许多细胞类型中被证实，这些细胞类型显示出不可检测的核糖核苷酸还原酶蛋白M1。少数细胞类型与蛋白质M1免疫反应性也含有免疫反应性硫氧还蛋白。然而，在大多数细胞中，没有这样的共定位可以证明。含有核糖核苷酸还原酶亚基M1的细胞和硫氧还蛋白之间缺乏相关性表明硫氧还蛋白不是大鼠组织中核糖核苷酸还原酶的生理氢供体，并且两种酶的表达受到不同的调节。
The cellular distribution of thioredoxin and protein M1 of ribonucleotide reductase in adult rat tissues was investigated with immunohistochemical techniques using specific antisera. Tissues with high or low frequency of either mitotic or meiotic cell divisions were compared. Thioredoxin was demonstrated in many cells types that showed no detectable protein M1 of ribonucleotide reductase. A few cell types with protein M1 immunoreactivity also contained immunoreactive thioredoxin. However, in most cells no such co-localization could be demonstrated. This lack of correlation between cells containing subunit M1 of ribonucleotide reductase and the thioredoxin indicates that thioredoxin is not the physiological hydrogen donor for ribonucleotide reductase in rat tissues and that the expression of two enzymes is differently regulated.