Ex vivo stability of the rodent-borne hantaan virus in comparison to that of arthropod-borne members of the Bunyaviridae family
Ex vivo stability of the rodent-borne hantaan virus in comparison to that of arthropod-borne members of the Bunyaviridae family
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DOI：
10.1128/aem.02869-06
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发表时间：
2007-04-01
影响因子：
4.4
通讯作者：
Lundkvist, A.
中科院分区：
文献类型：
--
作者：
Hardestam, J.;Simon, M.;Lundkvist, A.
病毒对不同传播途径的适应对环境中病毒稳定性的可能影响尚不清楚。在这项研究中，我们比较了布尼亚病毒科的三种病毒的稳定性：啮齿动物传播的汉坦病毒汉滩病毒(HTNV)、沙蝇传播的白喉病毒西西里病毒(SFSV)和壁虱传播的奈氏病毒克里米亚-刚果出血热病毒(CCHFV)。这些病毒的传播途径不同：SFSV和CCHFV是媒介传播的，而HTNV直接在宿主之间传播，并通过环境传播给人类。我们研究了这些病毒在宿主外的稳定性方面是否存在差异。分析了病毒在不同温度(4℃、20℃和37℃)下暴露并在20℃干燥后在不同时间点的存活情况。我们观察到在潮湿条件下的稳定性明显不同，特别是在4℃时，分别在528、96和15天后检测到传染性的SFSV、HTNV和CCHFV。这三种病毒对干燥同样敏感，如在铝制圆盘上干燥所示。此外，HTNV和SFSV在30%乙醇中能部分存活2分钟，而CCHFV则不能。保存在37℃直到失去感染力的HTNV、SSFSV和CCHFV的电子显微镜图像仍然显示出病毒粒子的出现，但与未孵育的样品相比，其形状和密度都异常。总之，我们的研究指出了布尼亚病毒科病毒在体外稳定性方面的重要差异。
The possible effect of virus adaptation to different transmission routes on virus stability in the environment is not well known. In this study we have compared the stabilities of three viruses within the Bunyaviridae family: the rodent-borne Hantavirus Hantaan virus (HTNV), the sand fly-borne Phlebovirus sandfly fever Sicilian virus (SFSV), and the tick-borne Nairovirus Crimean-Congo hemorrhagic fever virus (CCHFV). These viruses differ in their transmission routes: SFSV and CCHFV are vector borne, whereas HTNV is spread directly between its hosts, and to humans, via the environment. We studied whether these viruses differed regarding stability when kept outside of the host. Viral survival was analyzed at different time points upon exposure to different temperatures (4 degrees C, 20 degrees C, and 37 degrees C and drying at 20 degrees C. We observed clearly different stabilities under wet conditions, particularly at 4 degrees C, where infectious SFSV, HTNV, and CCHFV were detectable after 528, 96, and 15 days, respectively. All three viruses were equally sensitive to drying, as shown by drying on aluminum discs. Furthermore, HTNV and SFSV partially survived for 2 min in 30% ethanol, whereas CCHFV did not. Electron microscopy images of HTNV, SSFSV, and CCHFV stored at 37 degrees C until infectivity was lost still showed the occurrence of virions, but with abnormal shapes and densities compared to those of the nonincubated samples. In conclusion, our study points out important differences in ex vivo stability among viruses within the Bunyaviridae family.