A Mycoplasma protein homologous to mammalian SRP54 recognizes a highly conserved domain of SRP RNA.
A Mycoplasma protein homologous to mammalian SRP54 recognizes a highly conserved domain of SRP RNA.
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DOI：
10.1093/nar/20.21.5763
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发表时间：
1992-11
影响因子：
14.9
通讯作者：
T. Samuelsson
中科院分区：
文献类型：
--
作者：
T. Samuelsson
在丝状支原体中发现了一种与哺乳动物信号识别颗粒（SRP）亚基SRP 54同源的蛋白。支原体蛋白在大肠杆菌中表达并纯化至接近同质。它在体外显示出与具有与SRP的RNA组分相关的结构特征的小支原体RNA特异性结合。这些发现提供了支原体核糖核蛋白复合物的证据，让人联想到SRP。在SRP 54同源物的存在下，一部分RNA被保护免于核糖核酸酶消化。保护区包含在进化过程中在SRP RNA中高度保守的结构元件。
A protein homologous to SRP54, a subunit of the mammalian signal recognition particle (SRP), was identified in Mycoplasma mycoides. The mycoplasma protein was expressed in E.coli and purified to near homogeneity. It was shown to bind specifically in vitro to a small mycoplasma RNA with structural features related to the RNA component of SRP. These findings provide evidence of a ribonucleoprotein complex in mycoplasma reminiscent of SRP. A part of the RNA was protected from ribonuclease digestion in the presence of the SRP54 homologue. The protected region contains structural elements that have been highly conserved in SRP RNAs during evolution.