The effect of overexpression of two Brassica CBF/DREB1-like transcription factors on photosynthetic capacity and freezing tolerance in Brassica napus
The effect of overexpression of two Brassica CBF/DREB1-like transcription factors on photosynthetic capacity and freezing tolerance in Brassica napus
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DOI：
10.1093/pcp/pci165
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发表时间：
2005-09-01
影响因子：
4.9
通讯作者：
Singh, J
中科院分区：
文献类型：
--
作者：
Savitch, LV;Allard, G;Singh, J
研究了两种甘蓝型CBF/DREB 1类转录因子（BNCBF 5和17）在甘蓝型油菜（Brassica napus cv.韦斯特研究。除了形成组成型耐冻性和组成型积累COR基因mRNA外，BNCBF 5-和17-过表达植物还积累参与光合作用和叶绿体发育的基因的中等转录水平，如通过微阵列和北方分析鉴定的。这些转录因子包括参与叶绿体光合作用发育的GLK 1和GLK 2样转录因子、叶绿体基质亲环蛋白ROC 4（AtCYP 20 -3）、P-淀粉酶和丙糖-P/Pi转运蛋白。在这些变化的同时，增加光合效率和能力，色素池的大小，增加能力的卡尔文循环酶，淀粉和蔗糖的生物合成酶，以及糖酵解和草酰乙酸/苹果酸交换，表明BNCBF过表达部分模仿冷诱导的光合驯化组成。综上所述，这些结果表明，BNCBF/DREB 1在芸苔属植物中的过表达不仅导致了组成型耐冻性的增加，而且还部分调节了叶绿体发育，以增加光化学效率和光合能力。
The effects of overexpression of two Brassica CBF/ DREB1-like transcription factors (BNCBF5 and 17) in Brassica napus cv. Westar were studied. In addition to developing constitutive freezing tolerance and constitutively accumulating COR gene mRNAs, BNCBF5- and 17-overexpressing plants also accumulate moderate transcript levels of genes involved in photosynthesis and chloroplast development as identified by microarray and Northern analyses. These include GLK1- and GLK2-like transcription factors involved in chloroplast photosynthetic development, chloroplast stroma cyclophilin ROC4 (AtCYP20-3), P-amylase and triose-P/Pi translocator. In parallel with these changes, increases in photosynthetic efficiency and capacity, pigment pool sizes, increased capacities of the Calvin cycle enzymes, and enzymes of starch and sucrose biosynthesis, as well as glycolysis and oxaloacetate/malate exchange are seen, suggesting that BNCBF overexpression has partially mimicked cold-induced photosynthetic acclimation constitutively. Taken together, these results suggest that BNCBF/DREB1 overexpression in Brassica not only resulted in increased constitutive freezing tolerance but also partially regulated chloroplast development to increase photochemical efficiency and photosynthetic capacity.