Evidence for seed transmission and symptomless growth of Ramularia collo-cygni in barley (Hordeum vulgare)
Evidence for seed transmission and symptomless growth of Ramularia collo-cygni in barley (Hordeum vulgare)
复制标题
DOI：
10.1111/ppa.12162
复制
发表时间：
2014-08-01
期刊：
影响因子：
2.7
通讯作者：
Oxley, S. J. P.
中科院分区：
文献类型：
--
作者：
Havis, N. D.;Nyman, M.;Oxley, S. J. P.
青枯病(RCC)正成为欧洲大麦种植者的一个日益严重的问题。然而，这种病原体的生命周期只是缓慢地被阐明。在这项研究中，从1999年到2010年在一些收获的种子样本中检测到了RCC DNA，平均水平在2009年达到峰值。在对照试验和田间试验中，进行了一系列试验，以确定病原菌是否可以从大麦种子转移到幼苗，也可以从种子通过发育中的植株进入下一代种子。对种子的检测结果表明，该真菌在生长季节结束时在收获的谷物样品中广泛存在，并可从受感染的种子库传播给发育中的植物。对受感染的幼苗的检查没有发现孢子的存在，但在叶片中发现了真菌结构。对该真菌在种子中的位置进行了检测，在胚组织和非胚组织中都发现了RCC DNA。讨论了结实病原菌对大麦生产的影响。
Ramularia collo-cygni (Rcc) is becoming an increasing problem for barley growers across Europe. However, the life cycle of the pathogen is only slowly being elucidated. In this study, Rcc DNA was detected in a number of harvested seed samples from 1999 to 2010, with mean levels peaking in winter barley samples in 2009. A number of experiments were carried out to determine whether the pathogen could move from barley seed to seedlings, and also from seed through the developing plant and into the subsequent generation of seed, both in controlled experiments and in field trials. Results from testing of seed indicated that the fungus is widespread at the end of the growing season in harvested grain samples and can be transmitted to developing plants from infected seed stock. Examination of infected seedlings did not reveal the presence of spores but fungal structures were found within the leaf. The location of the fungus within seed was examined, with Rcc DNA found in both embryo and non-embryo tissue. The implications for barley production of the pathogen being seedborne are discussed.