ASSOCIATION OF THE LUPUS ANTIGEN LA WITH A SUBSET OF U6 SNRNA MOLECULES
ASSOCIATION OF THE LUPUS ANTIGEN LA WITH A SUBSET OF U6 SNRNA MOLECULES
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DOI：
10.1093/nar/13.7.2617
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
STEITZ, JA
中科院分区：
文献类型：
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作者：
RINKE, J;STEITZ, JA
U6单链RNA是一类主要的小RNA-蛋白质复合体，Sm单链RNPs存在于哺乳动物的细胞核中。在这里，我们报告了来自人类和小鼠细胞的相当大一部分U6RNA(约10%)与另一种狼疮抗原，50kd La蛋白有关。La结合的U6亚群以3‘端异质性和部分甲基化为特征。这些U6分子具有富含U的3‘末端，这可能是它们与La蛋白选择性结合的原因。讨论了它们是否是Sm SNRNP中发现的主要U6 RNAs的前体的问题。
U6 snRNA is a component of the major class of small RNA-protein complexes, the Sm snRNPs, present in mammalian cell nuclei. Here we report that a substantial fraction (about 10%) of U6 RNA from human and mouse cells is associated with another lupus antigen, the 50 kd La protein. The La-bound U6 subpopulation is characterized by 3′ end heterogeneity and partial undermethylation. These U6 molecules have U-rich 3′ termini that could be responsible for their selective association with the La protein. The question of whether they are precursors to the major U6 RNAs found in Sm snRNPs is discussed.