Developmental Expression of Calcitriol Receptors, 9-Kilodalton Calcium-Binding Protein, and Calcidiol 24-Hydroxylase in Human Intestine
Developmental Expression of Calcitriol Receptors, 9-Kilodalton Calcium-Binding Protein, and Calcidiol 24-Hydroxylase in Human Intestine
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人肠中骨化三醇受体、9 千道尔顿钙结合蛋白和骨化二醇 24-羟化酶的发育表达
作者：
E. Delvin;V. Lopez;E. Levy;D. Ménard
人肠粘膜由位于绒毛不同部位的处于持续更新和分化过程中的高度活跃的上皮细胞组成。隐窝含有大量的复制细胞，这些细胞在到达绒毛尖端时获得完全分化的状态。除了在骨细胞稳态中公认的作用外，钙三醇还在正常白细胞和骨髓白血病细胞的细胞分化和增殖中发挥作用。我们先前已经记录了特定骨化三醇受体在13-20周龄人胎儿的小肠和大肠细胞中的存在和分布，并且骨化三醇能够促进人肠上皮细胞在器官培养中的增殖或分化，这取决于胎龄。我们现在表明，而转录钙三醇受体是丰富的从十二指肠到结肠，那些9 kD的钙结合蛋白主要存在于十二指肠和空肠，并在较小程度上在回肠和结肠。25-羟基胆钙化醇-24-羟化酶的成绩单不能检测到在任何肠段，尽管长时间曝光的凝胶。免疫荧光染色的9-kD钙结合蛋白只观察到在小肠和结肠的上皮细胞，上皮下层总是阴性。9-kD钙结合蛋白在十二指肠、空肠和回肠中沿绒毛轴沿着呈梯度分布，从发育中的绒毛端到绒毛端逐渐增加。基于目前的观察和骨化三醇促进人胎儿增殖和分化的事实，肠细胞中骨化三醇受体和9-kD钙结合蛋白的转录本的存在开启了有趣的可能性，因为它们在子宫内人肠道发育和结肠直肠癌控制中的作用。
Human intestinal mucosa consists of highly active epithelial cells in continual renewal and differentiation processes located at different portions of the villi. The crypt contains abundant replicating cells which, upon reaching the villus tip, acquire their fully differentiated state. Besides its well recognized role in bone cell homeostasis, calcitriol has been attributed a role in cellular differentiation and proliferation in normal leukocytes and myeloid leukemia cells. We have previously documented the presence and the distribution of specific calcitriol receptors in the cells of the small and large intestine from 13-20-wk-old human fetuses and that calcitriol was able to promote human intestinal epithelium proliferation or differentiation, in organ culture, depending upon fetal age. We now show that, whereas transcripts for calcitriol receptors are abundant from duodenum to colon, those for the 9-kD calcium-binding protein are present mainly in the duodenum and the jejunum and to a lesser extent in the ileum and the colon. Transcripts for 25-hydroxycholecalciferol-24-hydroxylase could not be detected in any of the intestine segments despite a prolonged exposition of the gels. Immunofluorescence staining for the 9-kD calcium-binding protein was exclusively observed in the epithelial cells of the small intestine and colon, the subepithelial layers being always negative. The 9-kD calcium-binding protein distribution along the crypt-villus axis appeared as a gradient, increasing from the developing crypt to the tip of the villus in the duodenum, jejunum, and ileum. Based on the present observations and on the fact that calcitriol promotes human fetal proliferation and differentiation, the presence of transcripts for calcitriol receptors and 9-kD calcium-binding protein in the intestinal cell opens interesting possibilities as of their role in the in utero human gut development and the control of colorectal cancers.
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DOI：
--
发表时间：
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期刊：
The Journal of biological chemistry
影响因子：
--
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DOI：
10.1210/jcem.78.2.8106618
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作者：
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DOI：
10.1177/29.1.7009730
发表时间：
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期刊：
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影响因子：
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通讯作者：
Taylor,AN