lncRNA TNXA-PS1 Modulates Schwann Cells by Functioning As a Competing Endogenous RNA Following Nerve Injury
lncRNA TNXA-PS1 Modulates Schwann Cells by Functioning As a Competing Endogenous RNA Following Nerve Injury
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lncRNA TNXA-PS1 在神经损伤后作为竞争性内源 RNA 调节雪旺细胞
DOI：
10.1523/jneurosci.3790-16.2018
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发表时间：
2018-07-18
影响因子：
5.3
通讯作者：
Yu, Bin
中科院分区：
文献类型：
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作者：
Yao, Chun;Wang, Yaxian;Yu, Bin
雪旺细胞作为三叉神经节中主要的神经胶质细胞，在周围神经损伤修复中起着至关重要的作用。寻找一种有效的方法来促进雪旺细胞的激活可能会促进周围神经的修复。长非编码RNA(LncRNAs)调控基因表达，参与多种生物学过程。然而，LncRNAs在周围神经再生中的作用尚不完全清楚。在这项研究中，我们利用基因芯片获得了雄性大鼠坐骨神经损伤后的整体lncRNA，并进一步研究了其中一个受Dys调控的lncRNA，TNXA-PS1，证实了它在调节雪旺细胞中的重要作用。沉默TNAX-PS1可促进雪旺氏细胞的迁移，机制分析表明，TNXA-PS1可能通过海绵化miR-24-3p/miR-152-3p并影响双特异性磷酸酶1(DUSP1)的表达而发挥其调节作用。对大鼠神经损伤后坐骨神经节段的lncRNA表达谱分析表明，lncRNA TNXA-PS1是雪旺细胞迁移的关键调节因子，为神经损伤修复提供了潜在的治疗靶点。
As the major glia in PNS, Schwann cells play a critical role in peripheral nerve injury repair. Finding an efficient approach to promote Schwann cell activation might facilitate peripheral nerve repair. Long noncoding RNAs (lncRNAs) have been shown to regulate gene expression and take part in many biological processes. However, the role of lncRNAs in peripheral nerve regeneration is not fully understood. In this study, we obtained a global lncRNA portrayal following sciatic nerve injury in male rats using microarray and further investigated one of these dys-regulated lncRNAs, TNXA-PS1, confirming its vital role in regulating Schwann cells. Silencing TNAX-PS1 could promote Schwann cell migration and mechanism analyses showed that TNXA-PS1 might exert its regulatory role by sponging miR-24 - 3p/miR-152-3p and affecting dual specificity phosphatase 1 (Dusp1) expression. Systematic lncRNA expression profiling of sciatic nerve segments following nerve injury in rats suggested lncRNA TNXA-PS1 as a key regulator of Schwann cell migration, providing a potential therapeutic target for nerve injury repair.