The microRNA miR‐184 regulates the CYP303A1 transcript level to control molting of Locusta migratoria
The microRNA miR‐184 regulates the CYP303A1 transcript level to control molting of Locusta migratoria
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DOI：
10.1111/1744-7917.12837
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发表时间：
2020-06
期刊：
影响因子：
4
通讯作者：
Yan-li Wang;Lixian Wu;Huiyong Li;Xue Wen;E. Ma;K. Zhu;Jianzhen Zhang
中科院分区：
文献类型：
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作者：
Yan-li Wang;Lixian Wu;Huiyong Li;Xue Wen;E. Ma;K. Zhu;Jianzhen Zhang
细胞色素P450单加氧酶（CYP）在昆虫中具有重要的生理功能。CYP 303 A1在昆虫中高度保守，在飞蝗和果蝇成虫羽化过程中发挥着不可或缺的作用。然而，CYP 303 A1是如何被调节以控制昆虫发育过程的仍然没有研究。在这项研究中，我们在LmCYP 303 A1的编码序列中发现了miR-184的功能结合位点。荧光素酶报告基因检测结果表明，miR-184能够靶向LmCYP 303 A1并调控其在体外的表达。此外，通过向蝗虫显微注射agomir过量表达miR-184降低了LmCYP 303 A1的转录并导致异常蜕皮，这与通过向蝗虫直接注射dsLmCYP 303 A1沉默LmCYP 303 A1的表型相似。同时，通过注射阿托洛尔下调miR-184增加了LmCYP 303 A1的转录，并导致蜕皮缺陷。这些结果表明，miR-184可以靶向LmCYP 303 A1来调节L. migratoria，这可能被认为是一个新的目标害虫控制。
Cytochrome P450 monooxygenases (CYPs) play essential physiological functions in insects. CYP303A1 is highly conserved in insect species studied to date, and shows an indispensable role for adult eclosion in both Locusta migratoria and Drosophila melanogaster. However, how CYP303A1 is regulated to control insect developmental processes remains uninvestigated. In this study, we discovered functional binding sites for miR‐184 in the coding sequence of LmCYP303A1. The luciferase reporter assay showed that miR‐184 could target LmCYP303A1 and regulate its expression in vitro. Furthermore, overexpression of miR‐184 through microinjection of agomir to locusts reduced the transcripts of LmCYP303A1 and led to abnormal molting, which is similar to the phenotype of silencing LmCYP303A1 by direct injection of dsLmCYP303A1 to locusts. Meanwhile, down‐regulation of miR‐184 by injection of antagomir increased the LmCYP303A1 transcript and caused molting defects. These findings suggested that miR‐184 could target LmCYP303A1 to regulate the molting process in L. migratoria, which might be considered as a novel target for pest control.