A model system for analyzing somatic mutations in Drosophila melanogaster
A model system for analyzing somatic mutations in Drosophila melanogaster
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DOI：
10.1038/nmeth1027
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发表时间：
2007-05-01
期刊：
影响因子：
48
通讯作者：
Vijg, Jan
中科院分区：
文献类型：
--
作者：
Garcia, Ana Maria;Derventzi, Anastasia;Vijg, Jan
目前还没有比较不同脊椎动物和无脊椎动物之间体细胞突变频率和谱的好方法。在这里，我们描述了一种新的在黑腹葡萄球菌中的LacZ突变报告系统，它是对小鼠现有系统的补充。用新模型获得的结果表明，自发突变的频率是小鼠的两到三倍，大多数突变的特征是大的基因组重排。
Presently there are no good assays for comparing somatic mutation frequencies and spectra between different vertebrate and invertebrate organisms. Here we describe a new lacZ mutation reporter system in D. melanogaster, which complements existing systems in the mouse. The results obtained with the new model indicate two- to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements.