PpeTAC1 promotes the horizontal growth of branches in peach trees and is a member of a functionally conserved gene family found in diverse plants species
PpeTAC1 promotes the horizontal growth of branches in peach trees and is a member of a functionally conserved gene family found in diverse plants species
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DOI：
10.1111/tpj.12234
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发表时间：
2013-08-01
期刊：
影响因子：
7.2
通讯作者：
Scorza, Ralph
中科院分区：
文献类型：
--
作者：
Dardick, Chris;Callahan, Ann;Scorza, Ralph
树木具有巨大的建筑可塑性，使它们能够在高度竞争的环境下最大限度地暴露在阳光下。树木结构的一个关键组成部分是树枝角度，但人们对树木树枝空间图案的分子基础知之甚少。在这里，我们报告了桃树中 br 突变的候选基因的鉴定，该突变与垂直方向的分支生长有关，称为“支柱”或“bromy”。 Ppa010082，注释为桃基因组序列中的假设蛋白，使用下一代基于序列的作图方法被鉴定为 br 的候选基因。序列相似性搜索将水稻 TAC1（分蘖角控制 1）确定为推定的直系同源物，因此我们将其命名为 PpeTAC1。在单子叶植物中，TAC1 已知会通过增加分蘖角度来导致生长不太紧凑。在拟南芥中，attac1突变体表现出更多的垂直分枝生长角度，表明该基因普遍具有促进分枝水平生长的功能。 TAC1 基因属于所有植物基因组中发现的一个基因家族（此处称为 IGT，因为有一个共享的保守基序），该家族由两个分支组成：一个包含 TAC1 样基因；另一个包含 TAC1 样基因。另一个包含 LAZY1，其中包含 EAR 基序，并通过影响极性生长素运输促进水稻和拟南芥的垂直芽生长。数据表明，IGT 基因很古老，并且在决定植物芽生长角度方面发挥着保守的作用。了解 IGT 基因如何调节分支角度将有助于了解陆生植物不同的结构生长习性如何进化。
Trees are capable of tremendous architectural plasticity, allowing them to maximize their light exposure under highly competitive environments. One key component of tree architecture is the branch angle, yet little is known about the molecular basis for the spatial patterning of branches in trees. Here, we report the identification of a candidate gene for the br mutation in Prunus persica (peach) associated with vertically oriented growth of branches, referred to as pillar' or broomy'. Ppa010082, annotated as hypothetical protein in the peach genome sequence, was identified as a candidate gene for br using a next generation sequence-based mapping approach. Sequence similarity searches identified rice TAC1 (tiller angle control 1) as a putative ortholog, and we thus named it PpeTAC1. In monocots, TAC1 is known to lead to less compact growth by increasing the tiller angle. In Arabidopsis, an attac1 mutant showed more vertical branch growth angles, suggesting that the gene functions universally to promote the horizontal growth of branches. TAC1 genes belong to a gene family (here named IGT for a shared conserved motif) found in all plant genomes, consisting of two clades: one containing TAC1-like genes; the other containing LAZY1, which contains an EAR motif, and promotes vertical shoot growth in Oryza sativa (rice) and Arabidopsis through influencing polar auxin transport. The data suggest that IGT genes are ancient, and play conserved roles in determining shoot growth angles in plants. Understanding how IGT genes modulate branch angles will provide insights into how different architectural growth habits evolved in terrestrial plants.