Expression profile of calcium-dependent protein kinase (CDPKs) genes during the whole lifespan and under phytohormone treatment conditions in rice (Oryza sativa L. ssp indica)
Expression profile of calcium-dependent protein kinase (CDPKs) genes during the whole lifespan and under phytohormone treatment conditions in rice (Oryza sativa L. ssp indica)
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DOI：
10.1007/s11103-009-9475-0
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发表时间：
2009-06-01
影响因子：
5.1
通讯作者：
Lin, Yongjun
中科院分区：
文献类型：
--
作者：
Ye, Shuifeng;Wang, Lei;Lin, Yongjun
钙依赖性蛋白激酶（Ca2 + dependent protein kinases，CDPKs）通过调节Ca2+信号途径调控植物的生长发育和对各种逆境的响应。通过对水稻基因组的HMM图谱进行全基因组搜索，已在水稻基因组中鉴定出31个CDPK基因。本研究利用Affyestry水稻基因组芯片分析了该基因家族在明恢63、珍汕97及其杂交组合汕优63中的表达。本研究以水稻全生育期的27个组织为材料，沿着用3种激素（GA_3、NAA和KT）处理三叶期幼苗。所有31个基因被发现在至少一个研究的实验阶段表达，并揭示了不同的表达模式。我们鉴定了OsCPK基因在所有三个品种的雄蕊（开花前1天）、穗（抽穗期）、胚乳（授粉后数天）以及愈伤组织中的差异表达。结果表明，OsCPK2、OsCPK11、OsCPK14、OsCPK22、OsCPK25、OsCPK26、OsCPK27和OsCPK29等8个基因在穗部和雄蕊中呈优势表达，OsCPK6、OsCPK7、OsCPK12、OsCPK23和OsCPK31等5个基因在胚乳中表达上调。OsCPK基因在不同激素处理条件下的水稻幼苗中也受到调控，但其表达量在不同品种中并不相同。这些不同的表达谱触发了水稻中CDPK家族的功能分析。
Calcium-dependent protein kinases (CDPKs) control plant development and response to various stress environments through the important roles in the regulation of Ca2+ signaling. Thirty-one CDPK genes have been identified in the rice genome by a complete search of the genome based upon HMM profiles. In this study, the expression of this gene family was analyzed using the Affymetrix rice genome array in three rice cultivars: Minghui 63, Zhenshan 97, and their hybrid Shanyou 63 independently. Twenty-seven tissues sampled throughout the entire rice life-span were studied, along with three hormone treatments (GA3, NAA and KT), applied to the seedling at the trefoil stage. All 31 genes were found to be expressed in at least one of the experimental stages studied and revealed diverse expression patterns. We identified differential expression of the OsCPK genes in the stamen (1 day before flowering), the panicle (at the heading stage), the endosperm (days after pollination) and also in callus, in all three cultivars. Eight genes, OsCPK2, OsCPK11, OsCPK14, OsCPK22, OsCPK25, OsCPK26, OsCPK27 and OsCPK29 were found dominantly expressed in the panicle and the stamen, and five genes, OsCPK6, OsCPK7, OsCPK12, OsCPK23 and OsCPK31 were up-regulated in the endosperm stage. The OsCPK genes were also found to be regulated in rice seedlings subjected to different hormone treatment conditions, however their expression were not the same for all varieties. These diverse expression profiles trigger the functional analysis of the CDPK family in rice.