Altered interferon-γ response in patients with Q-fever fatigue syndrome
Altered interferon-γ response in patients with Q-fever fatigue syndrome
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DOI：
10.1016/j.jinf.2016.01.004
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发表时间：
2016-04-01
影响因子：
28.2
通讯作者：
van Deuren, Marcel
中科院分区：
文献类型：
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作者：
Keijmel, Stephan P.;Raijmakers, Ruud P. H.;van Deuren, Marcel
目的：Q 热疲劳综合征 (QFS) 的免疫机制是否是其背后的原因尚不清楚。对于急性Q热，抗原特异性干扰素γ（IFNγ）反应可能是诊断的有用工具，而IFNγ/白细胞介素（IL）-2的产生比率可能是慢性Q热和治疗监测的标志物。在这里，我们探讨了 QFS 患者中特定的 IFNg 产生和 IFN γ/IL-2 比率。方法：在 QFS 患者 (n = 20) 的离体刺激全血中测试 IFN γ 和 IL-2 产生，并与之前在血清阳性对照 (n = 135) 和慢性 Q 热患者 (n = 28) 中测定的结果进行比较。此外，还确定了患者特征与 IFNg、IL-2 和 IFNγ/IL-2 比值之间的相关性。结果：QFS 患者较年轻 (p < 0.001)，但性别分布与血清阳性对照和慢性 Q 热患者相似。伯内特柯克斯体九英里刺激显示，QFS 中的 IFNg 产量（中位数 319.5 pg/ml）高于血清阳性对照（120 pg/ml，p < 0.01），但与慢性 Q 热（2846 pg/ml）相当。 IFN γ/IL-2 比率与血清阳性对照相似，但低于慢性 Q 热患者 (p < 0.01)。症状持续时间与 IL-2 产生呈正相关，与 IFN gamma/IL-2 比率呈负相关。结论：这些结果表明 QFS 中细胞介导的免疫发生了改变，并表明与慢性 Q 热中的免疫反应不同。 (C) 2016 年英国感染协会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: Whether immunological mechanisms underlie Q-fever fatigue syndrome (QFS) remains unclear. For acute Q-fever, the antigen-specific interferon-gamma (IFN gamma) response may be a useful tool for diagnosis, and the IFN gamma/interleukin(IL)-2 production ratio may be a marker for chronic Q-fever and treatment monitoring. Here we explored the specific IFNg production and IFN gamma/IL-2 ratio in QFS patients.Methods: IFN gamma and IL-2 production were tested in ex-vivo stimulated whole blood of QFS patients (n = 20), and compared to those previously determined in seropositive controls (n = 135), and chronic Q-fever patients (n = 28). Also, the correlation between patient characteristics and IFNg, IL-2, and IFN gamma/IL-2 ratio was determined.Results: QFS patients were younger (p < 0.001), but gender distribution was similar to seropositive controls and chronic Q-fever patients. Coxiella burnetii Nine Mile stimulation revealed a higher IFNg production in QFS (median 319.5 pg/ml) than in seropositive controls (120 pg/ml, p< 0.01), but comparable to chronic Q-fever (2846 pg/ml). The IFN gamma/IL-2 ratio was similar to that in seropositive controls, but lower than in chronic Q-fever patients (p < 0.01). Symptom duration was positively correlated with IL-2 production, and negatively correlated with the IFN gamma/IL-2 ratio.Conclusions: These results point to an altered cell-mediated immunity in QFS, and suggest a different immune response than in chronic Q-fever. (C) 2016 The British Infection Association. Published by Elsevier Ltd. All rights reserved.