Lactobacillus johnsonii L531 reduces pathogen load and helps maintain short-chain fatty acid levels in the intestines of pigs challenged with Salmonella enterica Infantis
Lactobacillus johnsonii L531 reduces pathogen load and helps maintain short-chain fatty acid levels in the intestines of pigs challenged with Salmonella enterica Infantis
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DOI：
10.1016/j.vetmic.2019.02.003
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发表时间：
2019-03-01
影响因子：
3.3
通讯作者：
Wang, Jiu-Feng
中科院分区：
文献类型：
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作者：
He, Ting;Zhu, Yao-Hong;Wang, Jiu-Feng
在本研究中，我们筛选了从临床健康断奶仔猪结肠中分离的乳酸杆菌菌株，以获得潜在的益生菌特性，并分离出乳酸杆菌。johnsonii L531，其在体外产生高水平的有益代谢物（丁酸、乙酸和乳酸）。我们还评估了这种产生代谢物的益生菌在治疗沙门氏菌中的功效。婴儿感染。口服L.结果表明，在仔猪肠道中接种约氏沙门氏菌L531可显著降低肠道沙门氏菌的定植水平，促进肠道沙门氏菌的清除，并能显著降低沙门氏菌在肠道中的定植率。婴儿移位至脾脏。用促进SCFAs的益生菌L预处理。johnsonii L531显著改善S.与仅用S. Infantis感染的仔猪相比，其体重增加显著更大，饲料转化率更好。婴儿这些数据提供了进一步的证据，SCFAs促进益生菌L。johnsonii L531处理可能是控制沙门氏菌感染和维持代谢稳态的合适的非抗生素替代方案，从而在关键断奶期增强仔猪的肠道健康。
In the current study, we screened Lactobacillus strains isolated from the colon of clinically healthy weaned piglets for potential probiotic properties and isolated Lactobacillus. johnsonii L531, which produced high levels of beneficial metabolites (butyric, acetic, and lactic acid) in vitro. We also evaluated the efficacy of this metabolites-producing probiotic in treating Salmonella. Infantis infection. Oral administration of L. johnsonii L531 to newly weaned piglets significantly decreased levels of Salmonella colonization in colonic and jejunal contents, accelerated the clearance of Salmonella in feces after infection, and reduced S. Infantis translocation to the spleen. Pretreatment with SCFAs-promoting probiotic L. johnsonii L531 significantly ameliorated the depletion of SCFAs induced by S. Infantis infection and led to significantly greater weight gain and better feed conversion ratios compared to piglets challenged only with S. Infantis. These data provide further evidence that SCFAs-promoting probiotic L. johnsonii L531 treatment could be a suitable nonantibiotic alternative for controlling Salmonella infection and maintaining metabolic homeostasis, thereby enhancing the gut health of piglets during the critical weaning period.