Identification of a rhodanese-like protein involved in thiouridine biosynthesis in Thermus thermophilus tRNA
Identification of a rhodanese-like protein involved in thiouridine biosynthesis in Thermus thermophilus tRNA
复制标题
嗜热栖热菌 tRNA 中参与硫尿苷生物合成的硫尿苷样蛋白的鉴定
DOI：
10.1002/1873-3468.12499
复制
发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
渡辺 公綱
中科院分区：
文献类型：
--
作者：
鴫 直樹;朝井真一;渡辺 公綱
在嗜热tRNA的54位上，硫原子掺入2-硫代核糖胸苷（s2T或5-甲基-2-硫代尿苷）是通过一个由许多蛋白质组成的复杂系统完成的，该系统赋予翻译系统热稳定性。我们鉴定了ttuD（tRNA-2-thiouridine D）作为嗜热栖热菌中合成s2T54的基因。Rhodanese样蛋白TtuD增强半胱氨酸脱硫酶的活性，并在体外接受半胱氨酸脱硫酶产生的过硫化物。TtuD还增强硫代羧基化TtuB的形成，硫代羧基化TtuB是tRNA硫转移酶TtuA的硫供体。由于半胱氨酸脱硫酶是合成s2T和其他含硫化合物的第一个酶，TtuD具有将硫流引导至s2T合成的作用。
Incorporation of a sulfur atom into 2‐thioribothymidine (s2T or 5‐methyl‐2‐thiouridine) at position 54 in thermophile tRNA is accomplished by an elaborate system composed of many proteins which confers thermostability to the translation system. We identifiedttuD(tRNA‐two‐thiouridine D) as a gene for the synthesis of s2T54 inThermus thermophilus. The rhodanese‐like protein TtuD enhances the activity of cysteine desulfurases and receives the persulfide generated by cysteine desulfurasesin vitro. TtuD also enhances the formation of thiocarboxylated TtuB, the sulfur donor for the tRNA sulfurtransferase TtuA. Since cysteine desulfurases are the first enzymes in the synthesis of s2T and other sulfur‐containing compounds, TtuD has a role to direct sulfur flow to s2T synthesis.