Mesenchymal-to-epithelial transition in the placental tissues of patients with preeclampsia
Mesenchymal-to-epithelial transition in the placental tissues of patients with preeclampsia
复制标题
先兆子痫患者胎盘组织的间充质到上皮细胞的转变
DOI：
10.1038/hr.2016.97
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发表时间：
2017-01-01
影响因子：
5.4
通讯作者：
Chen, Dunjin
中科院分区：
文献类型：
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作者：
Du, Lili;Kuang, Liyun;Chen, Dunjin
在人类妊娠早期，间充质-上皮转化（MET）有助于子宫内膜间质细胞的蜕膜化。蜕膜化缺陷可干扰胎盘形成，并可导致妊娠并发症，包括先兆子痫。然而，子痫前期胎盘组织中的MET标记物尚未被表征。为了研究MET变化与先兆子痫之间的关系，我们评估了先兆子痫胎盘组织相对于正常胎盘的MET标志物。胎盘收集自20名先兆子痫和20名血压正常的健康妇女。MET相关标志物，包括E-cadherin、N-cadherin（神经钙粘蛋白）、波形蛋白、ZO-1（闭合细胞1）和SLUG，分别通过蛋白质印迹和定量实时PCR（Q-PCR）分析蛋白和mRNA水平。通过免疫组织化学方法，E-和N-钙粘蛋白定位于胎盘。Q-PCR和Western blot检测GLI 1和GLI 2的mRNA和蛋白表达。在先兆子痫胎盘中，E-cadherin和ZO-1的mRNA和蛋白水平相对于对照组升高，而N-cadherin、SLUG和波形蛋白的水平较低。E-cadherin和N-cadherin的染色强度与Western blot检测的蛋白水平一致。GLI 1和GLI 2的mRNA和蛋白水平在先兆子痫胎盘中显著低于对照胎盘。我们的结论是，MET在胎盘可能与子痫前期的进展。
During early pregnancy in humans, mesenchymal-to-epithelial transition (MET) contributes to decidualization of endometrial stromal cells in the uterus. Defects in decidualization can interfere with placental formation and can lead to pregnancy complications, including preeclampsia. However, MET markers in preeclamptic placental tissues have not been characterized. To investigate the association between changes in MET and preeclampsia, we evaluated MET markers in preeclamptic placental tissues relative to normal placentas. Placentas were collected from 20 preeclamptic and 20 normotensive healthy women. Protein and mRNA levels of MET-related markers, including E-cadherin, N-cadherin (neural cadherin), vimentin, ZO-1 (zona occludens 1) and SLUG, were analyzed via western blot and quantitative real-time PCR (Q-PCR), respectively. E-and N-cadherin were localized in the placentas through immunohistochemistry. The mRNA and protein expressions of GLI1 and GLI2 were detected by Q-PCR and western blot. In preeclamptic placentas, the mRNA and protein levels of E-cadherin and ZO-1 were elevated relative to the controls, whereas the levels of N-cadherin, SLUG and vimentin were lower. The staining intensities of E-and N-cadherin were consistent with their protein levels detected by western blot. The mRNA and protein levels of GLI1 and GLI2 were significantly lower in preeclamptic placentas compared with that in control placentas. We conclude that MET in the placenta may be associated with the progression of preeclampsia.