Determination of boldenone sulfoconjugate and related steroid sulfates in equine urine by high-performance liquid chromatography/tandem mass spectrometry.
Determination of boldenone sulfoconjugate and related steroid sulfates in equine urine by high-performance liquid chromatography/tandem mass spectrometry.
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通过高效液相色谱/串联质谱法测定马尿中的宝丹酮磺基结合物和相关类固醇硫酸盐。
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
J. Henion
中科院分区：
文献类型：
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作者：
Lars Weidolf;Edgar D. Lee;J. Henion
采用微孔高效液相色谱法分离磺基合成代谢类固醇。将洗脱液通过以负离子模式操作的离子喷雾液相色谱/质谱仪接口引入到三重四极杆质谱仪的大气压离子源中。检测限为10 pg的选择离子监测的分子离子和响应线性增加的浓度范围内的2.4个数量级。后处理的马尿液样品的液-固萃取程序，全扫描的子离子光谱的硫酸勃地农可以获得长达17天后，治疗剂量的十一碳烯酸勃地农的马。
Sulfoconjugated anabolic steroids were separated by micro-bore high-performance liquid chromatography. The eluent was introduced into the atmospheric pressure ion source of the triple-quadrupole mass spectrometer via an ion spray liquid chromatograph/mass spectrometer interface operated in the negative ion mode. The limit of detection was 10 pg on-column by selected ion monitoring of the molecular ion and the response increased linearly over a concentration range of 2.4 orders of magnitude. Following work-up by a liquid-solid extraction procedure of equine urine samples, full-scan daughter ion spectra of boldenone sulfate could be obtained up to 17 days after a therapeutic dose of boldenone undecylenate to a horse.