DESIGN OF IMMOBILE NUCLEIC-ACID JUNCTIONS
DESIGN OF IMMOBILE NUCLEIC-ACID JUNCTIONS
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DOI：
10.1016/s0006-3495(83)84292-1
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发表时间：
1983-01-01
影响因子：
3.4
通讯作者：
KALLENBACH, NR
中科院分区：
文献类型：
--
作者：
SEEMAN, NC;KALLENBACH, NR
相互作用以产生其中3个或更多个双螺旋从单个点发出的结构的核酸被称为形成连接。这种结构作为DNA复制和重组的中间体自然产生。已经提出，可以通过合成可以通过最大化沃森-克里克互补性缔合的限定序列的寡核苷酸组来产生稳定的连接。为了能够设计将形成特定结构的接合点的分子，提出了用于生成优化2个基本性质的核酸序列的有效算法：保真度和稳定性。保真度是指相对于所有替代配对结构形成连接复合物的相对概率。计算描述，允许近似预测的路口复合物的熔解曲线。
Nucleic acids that interact to generate structures in which 3 or more double helices emanate from a single point are said to form a junction. Such structures arise naturally as intermediates in DNA replication and recombination. It has been proposed that stable junctions can be created by synthesizing sets of oligonucloetides of defined sequence that can associate by maximizing Watson-Crick complementarity. To make it possible to design molecules that will form junctions of specific architecture, an efficient algorithm is presented for generating nucleic acid sequences that optimize 2 fundamental properties: fidelity and stability. Fidelity refers to the relative probability of forming the junction complex relative to all alternative paired structures. Calculations are described that permit approximate prediction of the melting curves for junctions complexes.