Transcription Factor SP2 Regulates Ski-mediated Astrocyte Proliferation In Vitro
Transcription Factor SP2 Regulates Ski-mediated Astrocyte Proliferation In Vitro
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DOI：
10.1016/j.neuroscience.2021.10.013
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Haihong Zhang
中科院分区：
文献类型：
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作者：
Chaoming Da;Haiyang Liao;Haihong Zhang
ranscription factors bind specific sequences upstream of the 50end of their target genes to ensureproper spatiotemporal expression of the target gene. This study aims to demonstrate that the transcription factorSP2 regulates expression of the Ski gene, which has specific binding sites for SP2, and thus enables Ski to reg-ulate astrocyte proliferation. The upstream regulation mechanism of astrocyte proliferation was explored to fur-ther regulate the formation of glial scar in specific time and space after spinal cord injury. JASPAR and UCSCdatabases were used to predict transcription factor binding and the threshold was gradually reduced to screentranscription factors upstream of Ski, leading to the identification of SP2. Next, we analyzed the correlationbetween the expression of SP2 and Ski in normal astrocytes and reactive astrocytes, as well as the changes inastrocyte proliferation. To confirm that SP2 regulates Ski during astrocyte proliferation, astrocytes were trans-fected siRNA targeting SP2 and then astrocyte proliferation were analyzed. Finally, a dual luciferase reporterassay and Chromatin immunoprecipitation (ChIP) assay confirmed that the promoter region of Ski contained aspecific SP2 binding site. This is the first that SP2 has been identified and confirmed to play an important rolein astrocyte proliferation by regulating Ski expression. These results may help identify novel targets for the treat-ment of spinal cord injury.
