Effects of chitosan oligosaccharides postharvest treatment on the quality and ripening related gene expression of cultivated strawberry fruits
Effects of chitosan oligosaccharides postharvest treatment on the quality and ripening related gene expression of cultivated strawberry fruits
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DOI：
10.3233/jbr-180307
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发表时间：
2019-01-01
影响因子：
1.7
通讯作者：
Yin, Heng
中科院分区：
文献类型：
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作者：
He, Yanqiu;Bose, Santosh Kumar;Yin, Heng
背景：壳寡糖(COS)是由壳聚糖降解而来的一种高效植物免疫激发剂，具有环保、易溶于水等优点，并能影响多种次生代谢物的含量，从而改善果实品质。方法：以50 mg·L-1和100 mg·L-1的蒸馏水为溶媒。然后将水果浸泡在COS溶液中，对照组浸泡在等量蒸馏水中1min。结果：CoS处理显著延缓了果实硬度、总糖和抗坏血酸含量的下降。此外，COS处理对维持较高的总酚浓度、花色苷含量和抗氧化活性有积极作用。此外，COS处理对果胶裂解酶(FAPL)、果胶酯酶(FAPE)和内切葡聚糖酶(FaEG)以及乙烯生物合成(FaACS和FaACO)途径等细胞壁多糖降解途径的相关基因表达也有抑制作用。这些结果表明，采后使用100 mg·L-1的COS处理有利于保持草莓果实的品质和贮藏寿命。结论：COS处理通过抑制草莓贮藏过程中的基因表达，有利于保持草莓果实的品质，维持较低的细胞壁降解活性和乙烯生物合成途径。
BACKGROUND: Chitosan oligosaccharide (COS), degraded from chitosan, has been proved as an effective plant immunity elicitor, eco-friendly, easily soluble in water and influenced several secondary metabolites content to improve fruit qualities.OBJECTIVE: The effects of chitosan oligosaccharides (COS) on the quality and ripening related gene expression of strawberry (Fragariaxananassa cv. qingxiang) during storage were investigated in this study.METHODS: COS was dissolved in distilled water at a concentration of 50 and 100 mg.L-1. Then the fruits were dipped in COS solution and the control group was dipped in equal amount of distilled water for 1 minute. After that the fruits were air-dried and stored at 20 +/- 0.5 degrees C with 80 +/- 2% relative humidity.RESULTS: COS treated fruits significantly delayed the loss of hardness, total sugar and ascorbate content. In addition, COS treatments had positive effect on maintaining higher concentration of total phenol, anthocyanin content and antioxidant activity. Moreover, COS treatments also had positive effect on inhibition of relative gene expression of cell wall polysaccharides degradation pathways, including pectate lyase (FaPL), pectin esterase (FaPE) and endoglucanase (FaEG) and ethylene biosynthesis (FaACS and FaACO) pathway. These findings suggest that the use of COS 100 mg.L-1 postharvest treatment is useful for maintaining quality and storage life of strawberry fruits.CONCLUSION: COS postharvest treatment appeared to have a beneficial impact on the quality retention, maintained lower activities of cell wall degradation and the ethylene biosynthesis pathway by suppressing the gene expression during storage of strawberry.