Magnetic Resonance Microscopy of the Adult Zebrafish
Magnetic Resonance Microscopy of the Adult Zebrafish
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DOI：
10.1089/zeb.2006.3.431
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发表时间：
2006-01-01
期刊：
影响因子：
2
通讯作者：
De Groot, Huub J. M.
中科院分区：
文献类型：
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作者：
Kabli, Samira;Alia, A.;De Groot, Huub J. M.
磁共振显微镜 (MRM) 是一种成像方式，可以在完整的不透明动物中无创采集高分辨率图像。斑马鱼（Danio rerio）是脊椎动物生物学研究的重要模式生物。然而，由于斑马鱼幼年和成年阶段的不透明性，斑马鱼的光学体内研究仅限于非常早期的发育阶段。高分辨率 MRM 的应用尚未在成年斑马鱼中进行探索。在这项研究中，我们应用并优化了高分辨率 MRM 方法来非侵入性地检查成年斑马鱼的解剖结构。通过T-2加权自旋回波和快速采集以及松弛增强快速采集（RARE）序列获得了清晰的形态质子图像，在9.4 T的磁场强度下揭示了整个完整斑马鱼的许多解剖细节。此外，成年斑马鱼的活体成像揭示了足够的解剖细节。据我们所知，这是应用高分辨率 MRM 研究成年斑马鱼详细解剖结构的第一份报告。
Magnetic resonance microscopy (MRM) is an imaging modality that allows for noninvasive acquisition of high-resolution images in intact opaque animals. The zebrafish (Danio rerio) is an important model organism for the study of vertebrate biology. However, optical in vivo studies in zebrafish are restricted to very early developmental stages due to the opacity of the juvenile and adult stages. Application of high resolution MRM has not yet been explored in adult zebrafish. In this study we applied and optimized high resolution MRM methods to examine anatomical structures noninvasively in adult zebrafish. Clear morphological proton images were obtained by T-2-weighted spin echo and rapid acquisition with rapid acquisition with relaxation enhancement (RARE) sequences which revealed many anatomical details in the entire intact zebrafish at a magnetic field strength of 9.4 T. In addition, in vivo imaging of adult zebrafish revealed sufficient anatomical details. To our knowledge this is the first report of the application of high resolution MRM to study detailed anatomical structures in adult zebrafish.