Both NK sensitive and resistant mycoplasma free cell lines stimulate release of NKCF.
Both NK sensitive and resistant mycoplasma free cell lines stimulate release of NKCF.
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NK 敏感和耐药的无支原体细胞系都会刺激 NKCF 的释放。
DOI：
10.1007/978-1-4684-8326-0_13
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发表时间：
1985
影响因子：
--
通讯作者：
Bonavida,B
中科院分区：
文献类型：
--
作者：
Wright,SC;Bonavida,B
本实验室对NK细胞裂解机制的研究表明，自然杀伤细胞毒因子(NKCF)可能是NK细胞的裂解介质。我们的NK细胞裂解机制模型认为，在NK细胞与靶细胞结合后，靶细胞刺激效应器释放NKCF(1，2)。为了支持这一点，我们发现，在人类(3)或小鼠(4)中，富含NK细胞的效应细胞群体在与NK敏感的肿瘤刺激细胞共同培养时会产生NKCF。这项研究的目的是确定在诱导NKCF释放的水平上是否存在任何NK特异性。还进行了实验，以确定支原体感染肿瘤刺激细胞是否对小鼠或大鼠脾细胞产生细胞毒上清有影响。结果表明，NK敏感和耐药的无支原体细胞株均能刺激NKCF的释放。一些支原体感染的细胞株可以产生细胞毒上清，但目前尚不清楚这种裂解活性是由NKCF还是其他因素引起的。
Studies on the NK lytic mechanism in our laboratory have produced evidence that natural killer cytotoxic factors (NKCF) may be the lytic mediators. Our model for the NK lytic mechanism proposes that after binding of the NK cell to the target, the target stimulates the effector to release NKCF (1,2). In support of this, we have found that effector cell populations enriched for NK cells in either human (3) or mouse (4) produce NKCF during coculture with NK sensitive tumor stimulator cells. The objective of this investigation was to determine if there is any NK specificity at the level of induction of NKCF release. Experiments were also carried out to determine if mycoplasma infection of tumor stimulator cells had any effect on the production of cytotoxic supernatants by murine or rat spleen cells. It was found that both NK sensitive and resistant mycoplasma free cell lines could stimulate release of NKCF. Some mycoplasma infected cell lines could generate cytotoxic supernatants, however, it is not clear whether the lytic activity is due to NKCF or some other agent.