AN IMMUNOCHEMICAL ANALYSIS OF OXYTOCIN AND VASOPRESSIN PROHORMONE PROCESSING INVIVO
AN IMMUNOCHEMICAL ANALYSIS OF OXYTOCIN AND VASOPRESSIN PROHORMONE PROCESSING INVIVO
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DOI：
10.1016/0196-9781(88)90014-9
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发表时间：
1988-01-01
期刊：
影响因子：
3
通讯作者：
GAINER, H
中科院分区：
文献类型：
--
作者：
ALTSTEIN, M;WHITNALL, MH;GAINER, H
抗血清对部分处理，未酰胺化形式的AVP和OT的提出，其特征在于放射免疫分析和免疫细胞化学。这些抗体，和抗体，识别完全加工，酰胺化形式的AVP和OT，与各种分馏方法一起使用，以研究激素原的含量，部分加工和完全加工形式的AVP和OT在下丘脑-神经垂体系统的成年和胎儿（E21）大鼠。胎儿脑和垂体中的AVP和OT裂解水平均低于成人（分别为1 ~ 3个数量级），且脑和垂体中OT裂解的检测较AVP延迟。成人和胎儿中的Pro-AVP切割效率高（分别为99%和95%切割），导致形成完全加工的酰胺化形式的AVP，没有可检测的部分加工的肽。成体中的前OT加工非常相似（超过99%裂解），导致形成完全加工的酰胺化OT。然而，胎儿中的Pro-OT加工效率非常低且不完全，导致40%未加工的前体和C-末端延伸的未酰胺化中间体形式（OT-Gly、OT-Gly-Lys和OT-Gly-Lys-Arg）的积累。
Antisera against partially processed, unamidated forms of AVP and OT were raised and characterized by radioimmunoassay and immunocytochemistry. These antibodies, and antibodies that recognize fully processed, amidated forms of AVP and OT, were used together with various fractionation methods to study the content of prohormones, partially processed and fully processed forms of AVP and OT in the hypothalamo-neurohypophysial system of adult and fetal (E21) rats. The levels of cleaved AVP and OT in the fetus were lower than those of the adult (1 to 3 orders of magnitude for brain and pituitary, respectively), and the detection of cleaved OT in brain and pituitary was delayed compared to that of AVP. Pro-AVP cleavage efficiency in the adult and the fetus was high (99 and 95% cleavage, respectively) resulting in formation of fully processed amidated forms of AVP, with no detectable partially processed peptides. Pro-OT processing in the adult was very similar (over 99% cleavage) resulting in formation of fully processed amidated OT. However, Pro-OT processing efficiency in the fetus was very low and incomplete, resulting in 40% unprocessed precursor and the accumulation of C-terminally extended unamidated intermediate forms (OT-Gly, OT-Gly-Lys, and OT-Gly-Lys-Arg).