Dramatic intraspecimen variations within the injected venom of Conus consors: An unsuspected contribution to venom diversity
Dramatic intraspecimen variations within the injected venom of Conus consors: An unsuspected contribution to venom diversity
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DOI：
10.1016/j.toxicon.2010.02.025
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发表时间：
2010-07-01
期刊：
影响因子：
2.8
通讯作者：
Favreau, Philippe
中科院分区：
文献类型：
--
作者：
Dutertre, Sebastien;Biass, Daniel;Favreau, Philippe
随着高灵敏度质谱技术的出现，可以对活体动物产生的微量各种分泌物进行研究，达到前所未有的详细程度。在本研究中，我们使用 LC-ESI-MS 分析了印度太平洋鱼食锥蜗牛 Conus consors 的注射毒液。虽然对几个圈养标本的长期跟踪揭示了该物种的典型“毒液指纹”，但也观察到了巨大的变化。在最极端的情况下，一只锥形蜗牛出乎意料地产生了两种截然不同的毒液特征，其中包含完全不同的肽组，且检测到的质量没有重叠。令人惊讶的是，毒液管中产生的肽与挤奶活锥螺后获得的肽之间没有相关性，这意味着选择和调节机制尚不清楚。我们的研究定义了样本内变异的概念，并证明了这种现象如何影响整体毒液多样性。 (C) 2010 Elsevier Ltd. 保留所有权利。
With the advent of highly sensitive mass spectrometry techniques, the minute amount of various secretions produced by living animals can be studied to a level of details never attained before. In this study, we used LC-ESI-MS to analyse the injected venom of an indo-pacific piscivorous cone snail, Conus consors. While long-term follow up of several captive specimens have revealed a typical "venom fingerprint" for this species, dramatic variations were also observed. In the most extreme case, a single cone snail unexpectedly produced two very distinct venom profiles containing completely different sets of peptides with no overlap of detected masses. Surprisingly, there was no correlation between the peptides produced in the venom duct and those obtained after milking live cone snails, implying yet unknown mechanisms of selection and regulation. Our study defines the notion of intra-specimen variation and demonstrates how this phenomenon contributes to the overall venom diversity. (C) 2010 Elsevier Ltd. All rights reserved.