Oxidation of Lactate to Carbon Dioxide by Rainbow Trout (Salmo gairdneri) Tissues
Oxidation of Lactate to Carbon Dioxide by Rainbow Trout (Salmo gairdneri) Tissues
复制标题
虹鳟鱼 (Salmo gairdneri) 组织将乳酸氧化为二氧化碳
DOI：
--
复制
发表时间：
1972
期刊：
影响因子：
--
通讯作者：
R. Jonas
中科院分区：
文献类型：
--
作者：
E. Bilinski;R. Jonas
以虹鳟（萨尔莫gairdneri）为材料，比较研究了不同组织进行不同阶段乳酸氧化的能力。在15 ℃下，通过测量14 CO2的形成，测定来自白色肌肉、红色侧线肌肉、心脏、肝脏、肾脏和鳃的组织切片对Na-L-乳酸盐-1-14 C（5 mM）和Na-L-乳酸盐-3-14 C（5 mM）的氧化速率。 在所有组织中，乳酸盐-1-14 C的14 CO2释放显著高于乳酸盐-3-14 C。肝脏是乳酸-1-14 C氧化最活跃的组织（在15 ℃下为2805毫微摩尔/克湿组织/小时），鳃是乳酸-3-14 C氧化最活跃的组织（在15 ℃下为556毫微摩尔/克湿组织/小时）。 对于这两种底物，白色肌肉中的活性非常有限，而其他组织具有中等水平的活性。结果表明，在鳟鱼，乳酸的catenorization可能发生通过丙酮酸的氧化脱羧和肝脏在这样的过程中起着重要的作用。看来，这也是一个完整的…
A comparative study on the ability of various fish tissues to carry out different stages of lactate oxidation was conducted with rainbow trout (Salmo gairdneri). Rate of oxidation of Na-L-lactate-1-14C (5 mM) and Na-L-lactate-3-14C (5 mM) by tissue slices from white muscle, red lateral line muscle, heart, liver, kidney, and gills was determined at 15 C by measuring the formation of 14CO2. In all tissues the liberation of 14CO2 was considerably higher with lactate-1-14C than with lactate-3-14C. Liver was the most active tissue for oxidation of lactate-1-14C (2805 mμmoles/g wet tissue/hr at 15 C) and gills for oxidation of lactate-3-14C (556 mμmoles/g wet tissue/hr at 15 C). With both substrates activity in the white muscle was very limited, whereas other tissues had an intermediate level of activity. The results suggest that, in trout, the catabolism of lactate may take place through oxidative decarboxylation of pyruvate and that liver plays an important role in such a process. It appears also that complet...