Dynorphin and vasopressin: common localization in magnocellular neurons.
Dynorphin and vasopressin: common localization in magnocellular neurons.
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强啡肽和加压素：大细胞神经元中的常见定位。
DOI：
10.1126/science.6121376
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
vanwimersmaGriedanus,TB
中科院分区：
文献类型：
--
作者：
Watson,SJ;Akil,H;Fischli,W;Goldstein,A;Zimmerman,E;Nilaver,G;vanwimersmaGriedanus,TB
阿片肽啡肽广泛分布于大鼠的神经组织中。通过免疫细胞化学方法，先前已经表明，在垂体后叶和下丘脑神经分泌大细胞核的细胞中存在动力啡样免疫反应性，这些细胞也负责催产素、加压素及其神经素的合成。通过使用亲和纯化的抗血清对肌啡肽分子的非脑啡肽部分，现在已经证明，肌啡肽和抗利尿激素发生在大鼠的同一下丘脑细胞中，而肌啡肽和催产素发生在不同的细胞中。纯合子Brattleboro大鼠（抗利尿激素缺乏）的大细胞神经元含有与催产素分离的促啡肽。因此，尽管发生在相同的细胞中，但促啡肽和抗利尿激素似乎是在不同的遗传控制下产生的，可能是由不同的前体产生的。
The opioid peptide dynorphin is widely distributed in neuronal tissue of rats. By immunocytochemical methods, it was shown previously that dynorphin-like immunoreactivity is present in the posterior pituitary and the cells of the hypothalamic neurosecretory magnocellular nuclei which also are responsible for the synthesis of oxytocin, vasopressin, and their neurophysins. By using an affinity-purified antiserum to the non-enkephalin part of the dynorphin molecule it has now been demonstrated that dynorphin and vasopressin occur in the same hypothalamic cells of rats, whereas dynorphin and oxytocin occur in separate cells. Homozygous Brattleboro rats (deficient in vasopressin) have magnocellular neurons that contain dynorphin separate from oxytocin. Thus dynorphin and vasopressin, although they occur in the same cells, appear to be under separate genetic control and presumably arise from different precursors.