Embryo Aggregation in Pig Improves Cloning Efficiency and Embryo Quality.
Embryo Aggregation in Pig Improves Cloning Efficiency and Embryo Quality.
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DOI：
10.1371/journal.pone.0146390
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Salamone DF
中科院分区：
文献类型：
--
作者：
Buemo CP;Gambini A;Moro LN;Hiriart MI;Fernández-Martín R;Collas P;Salamone DF
本研究通过测定猪胚胎发育过程中囊胚直径、细胞数、DNA片段化水平、多能性相关基因、细胞凋亡相关基因、滋养层相关基因和DNA甲基化相关基因的表达，分析了猪克隆胚胎聚集对体外胚胎发育和胚胎质量的影响。将无透明带的重构克隆胚胎在孔系统的孔中培养，每个微孔放置一个（1x非聚集组）或三个（3x组）胚胎。实验结果表明，三胚聚集可提高克隆猪胚胎的囊胚形成率和囊胚直径。与1x囊胚相比，3x聚集克隆囊胚中的DNA片段化水平显著降低。Oct 4、Klf 4、Igf 2、Bax和Dnmt 1转录物的水平在聚集的胚胎中显著更高，而Nanog水平不受影响。Cdx 2和Bcl-xl的转录物基本上不可检测。我们的研究表明，在猪胚胎聚集可能是有益的克隆胚胎发育和胚胎质量，通过减少细胞凋亡水平和改善细胞重编程。
In this study, we analyzed the effects of the cloned embryo aggregation on in vitro embryo development and embryo quality by measuring blastocyst diameter and cell number, DNA fragmentation levels and the expression of genes associated with pluripotency, apoptosis, trophoblast and DNA methylation in the porcine. Zona-free reconstructed cloned embryos were cultured in the well of the well system, placing one (1x non aggregated group) or three (3x group) embryos per microwell. Our results showed that aggregation of three embryos increased blastocyst formation rate and blastocyst diameter of cloned pig embryos. DNA fragmentation levels in 3x aggregated cloned blastocysts were significantly decreased compared to 1x blastocysts. Levels of Oct4, Klf4, Igf2, Bax and Dnmt 1 transcripts were significantly higher in aggregated embryos, whereas Nanog levels were not affected. Transcripts of Cdx2 and Bcl-xl were essentially non-detectable. Our study suggests that embryo aggregation in the porcine may be beneficial for cloned embryo development and embryo quality, through a reduction in apoptotic levels and an improvement in cell reprogramming.
影响因子：
1.8
作者：
Park SK;Roh S;Park JI
通讯作者：
Park JI