Effects of estradiol on measurements of conduit artery endothelial function after ischemia and reperfusion in premenopausal women
Effects of estradiol on measurements of conduit artery endothelial function after ischemia and reperfusion in premenopausal women
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DOI：
10.1139/cjpp-2015-0589
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发表时间：
2016-12-01
影响因子：
2.1
通讯作者：
Parker, John D.
中科院分区：
文献类型：
--
作者：
Luca, Mary Clare;Liuni, Andrew;Parker, John D.
在绝经前妇女中，卵巢类固醇被认为在预防心血管疾病中发挥作用。我们的目的是评估雌激素的月经周期变化是否可以改变人类对缺血再灌注（IR）损伤的反应。在一项双盲交叉研究中，对10名月经周期规律的健康绝经前妇女进行了研究。在月经周期的早期和晚期卵泡期，在IR（肱动脉缺血15分钟，再灌注15分钟）之前和之后，通过超声测量桡动脉的流量介导的扩张（FMD）。这些访视的顺序不是随机的。当雌二醇水平较低（148.4 +/-19.8 pmol/L）时，IR显著减弱卵泡早期的FMD（IR前：7.1%+/-1.0%; IR后：3.6%+/-1.0%，P = 0.01）。相反，当雌二醇水平较高（825.7 +/-85.8 pmol/L，与早期卵泡相比P <0.001）时，在晚期卵泡期IR后FMD得以保留（IR前：7.2%+/-0.9%; IR后：7.0%+/-0.8%，P = NS，P = 0.03）。雌二醇浓度与IR诱导的内皮功能障碍之间存在显著的负相关关系（即，IR后FMD变化）（r = 0.59，r（2）= 0.36，P <0.01）。这些发现首次在人类中证实了雌二醇血清浓度的周期性变化与内皮细胞对IR的反应之间的明确关系。
In premenopausal women, ovarian steroids are felt to play a role in the prevention of cardiovascular disease. We aimed to assess whether menstrual cycle variations in estrogen can modify the response to ischemia-reperfusion (IR) injury in humans. In an investigator-blinded crossover study, 10 healthy premenopausal women with regular menstrual cycles were studied. They had flow-mediated dilatation (FMD) measured by ultrasound in the radial artery before and after IR (15 min of brachial artery ischemia, 15 min of reperfusion) during both the early and late follicular phases of the menstrual cycle. The order of these visits was not randomized. IR significantly blunted FMD in the early follicular phase (pre-IR: 7.1% +/- 1.0%; post-IR: 3.6% +/- 1.0%, P = 0.01) when estradiol levels were low (148.4 +/- 19.8 pmol/L). Conversely, FMD was preserved after IR during the late follicular phase (pre-IR: 7.2% +/- 0.9%; post-IR: 7.0% +/- 0.8%, P = NS, P = 0.03 compared with early follicular) when estradiol levels were high (825.7 +/- 85.8 pmol/L, P < 0.001 compared with early follicular). There was a significant inverse relationship between estradiol concentration and IR-induced endothelial dysfunction (i.e., change in FMD after IR) (r = 0.59, r(2) = 0.36, P < 0.01). These findings demonstrate, for the first time in humans, a clear relationship between the cyclical changes in serum concentrations of estradiol and the endothelium's response to IR.